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This Article Accepted manuscript (PDF) All Versions of this Article: JOE-09-0016v1 201/3/407    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, S. Articles by Lin, H. Search for Related Content PubMed PubMed Citation Articles by Li, S. Articles by Lin, H.

S Li, Guangzhou, China
Y Zhang, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
Y Liu, Guangzhou, China
X Huang, School of Life Science, Southwest University, Chongqing, China
W Huang, Guangzhou, China
D Lu, Guangzhou, China
P Zhu, Guangzhou, China
Y Shi, Guangzhou, China
C Cheng, The Chinese University of Hong Kong, Shatin, Hong Kong
X Liu, guangzhou, China
H Lin, Guangzhou, China

Correspondence: Xiaochun Liu, Email: lsslxc{at}mail.sysu.edu.cn

Abstract

To ascertain the neuroendocrine function of the kisspeptin/GPR54 system in non-mammalian species, full-length cDNAs encoding for Kiss1 and Kiss2 as well as their putative cognate receptors GPR54a and GPR54b, were isolated from goldfish (Carassius auratus). The deduced protein sequences between Kiss1 and Kiss2 in goldfish share very low similarity, but their putative mature peptides (kisspeptin-10) are relatively conserved. RT-PCR analysis demonstrated that the goldfish kiss1 gene (gfkiss1) is highly expressed in the optic tectum thalamus, intestine, kidney and testis, while the goldfish kiss2 gene (gfkiss2) is mainly detected in the hypothalamus, telencephalon, optic tectum thalamus, adipose tissue, kidney, heart and gonads. The two receptor genes (gfgpr54a and gfgpr54b) are highly expressed in the brain regions including telencephalon, optic tectum thalamus and hypothalamus. Both mature goldfish kisspeptin-10 peptides (gfKiss1-10 and gfKiss2-10) are biologically active as they could functionally interact with the two goldfish receptors expressed in cultured eukaryotic cells to trigger the down-stream signaling pathways with different potencies. The actions of gfKiss1-10 and gfKiss2-10 on luteinizing hormone (LH) secretion were further investigated in vitro and in vivo. Intraperitoneal administration of gfKiss1-10 to sexually mature female goldfish could increase the serum LH levels. However, this peptide does not significantly influence LH release from goldfish pituitary cells in primary culture, indicating that the peptide does not exert its actions at the pituitary level. On the other hand, gfKiss2-10 appears to be a much less potent peptide as it exhibits no significant in vivo bioactivity and is also inactive on the primary pituitary cells.

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				A. E. Oakley, D. K. Clifton, and R. A. Steiner Kisspeptin Signaling in the Brain Endocr. Rev., October 1, 2009; 30(6): 713 - 743. [Abstract] [Full Text] [PDF]