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This Article Accepted manuscript (PDF) All Versions of this Article: JOE-08-0521v1 201/2/253    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Komrakova, M. Articles by Stuermer, E. Search for Related Content PubMed PubMed Citation Articles by Komrakova, M. Articles by Stuermer, E.

M Komrakova, Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany
C Werner, Institute of Animal Breeding and Genetics, University of Goettingen, Goettingen, Germany
M Wicke, Institute of Animal Breeding and Genetics, University of Goettingen, Goettingen, Germany
B Nguyen, Department of Endocrinology, University of Goettingen, Goettingen, Germany
S Sehmisch, Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany
M Tezval, Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany
K Stuermer, Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany
E Stuermer, Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany

Correspondence: Marina Komrakova, Email: komrakova{at}yahoo.com

Abstract

The effect of daidzein (D), 4-Methylbenzylidene camphor (4-MBC) or estradiol-17Beta-benzoate (E) on muscle of osteoporotic rats during fracture healing was studied. After performing a metaphyseal tibia osteotomy in 96 osteoporotic 5-month-old female Sprague-Dawley rats, they received daily 50 mg D, 200 mg 4-MBC or 0.4 mg E per kg BW, or soy free (SF) diet up to 36 and 72 days. Mitochondrial activity, fibre area, capillary density were analyzed in M. gastrocnemius. Callus bridging of fracture was observed in half of the rats after 36 days. By day 72, fracture was healed in most of the animals. State 3 mitochondrial respiration enhanced in E, 4-MBC and D groups vs. SF after 36 days (30, 32 and 32 vs. 23 pmol O₂^-1 mg^-1). After 72 days, in E group it was still at a higher level vs. SF (25, 23 and 21 vs. 20 pmol O₂^-1 mg^-1). Size of fast oxidative glycolytic (FOG) and fast glycolytic (FG) fibers, capillary density did not differ significantly between the groups, however, after 36 days increase in D and 4-MBC groups was detectable. FOG diameter was 64, 66, 68 and 58x10<sup>-6</sub> m and FG diameter was 88, 98, 95 and 89x10^-6 m in SF, D, 4-MBC and E groups. The ratio of capillaries to muscle fiber was 1.1, 1.4, 1.3, and 1.1 in SF, D, 4-MBC and E groups by day 36. Daidzein and 4-MBC react similar to estrogen thereby improving oxidative cell metabolism in severe osteoporotic rats. The level of mitochondrial activity was higher, though no significant morphological differences could be shown