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S Poncin, Morphologie Experimentale (MOEX), Universite catholique de Louvain (UCL), Brussel, Belgium
I Colin, Morphologie Experimentale (MOEX), Universite catholique de Louvain (UCL), Brussel, Belgium
A Gerard, Morphologie Experimentale (MOEX), Universite catholique de Louvain (UCL), Brussel, 1200, Belgium

Correspondence: Anne-Catherine Gerard, Email: anne-catherine.gerard{at}uclouvain.be

Abstract

In addition to reactive oxygen species (ROS) produced by mitochondria during aerobic respiration, thyrocytes are continuously producing H2O2, a key element for hormonogenesis. Because nothing is known about ROS implication in normal non stimulated cells, we studied their possible involvement in thyrocytes incubated with a potent antioxidant, N-acetylcysteine (NAC). NAC, which blocked the production of intracellular ROS, also decreased dual oxidases, thyroperoxidase, pendrin and thyroglobulin protein and/or gene expression. By contrast, Na+/I- symporter mRNA expression was unaffected. Among antioxidant systems, peroxiredoxin5 expression was reduced by NAC, whereas peroxiredoxin3 increased and catalase remained unchanged. In vivo, the expression of both dual oxidases and peroxiredoxin5 proteins was also decreased by NAC. In conclusion, when intracellular ROS levels drop below a basal threshold, the expression of proteins involved in thyroid cell function is hampered. This suggests that keeping ROS at a minimal level is required for safeguarding thyrocyte function.

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