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E Guillod-Maximin, NMPA, University Paris11, ORSAY, France
A Roy, NMPA, University Paris11, ORSAY, France
C Vacher, NMPA, University Paris11, ORSAY, France
A Aubourg, NMPA, University Paris11, ORSAY, France
V Bailleux, NMPA, University Paris11, ORSAY, France
A Lorsignol, INSERM, Toulouse, France
L Penicaud, INSERM, Toulouse, France
M Parquet, NMPA, University Paris11, ORSAY, France
M Taouis, NMPA, University Paris11, ORSAY, 91405, France

Correspondence: Mohammed Taouis, Email: mohammed.taouis{at}u-psud.fr

Abstract

Adiponectin is involved in the control of energy homeostasis in peripheral tissues through AdipoR1 and AdipoR2 receptors. Increasing evidences suggest that this adipocyte-secreted hormone may also act at the hypothalamic level to control energy homeostasis. In the present study, we observed the gene and protein expressions of AdipoR1 and AdipoR2 in rat hypothalamus using different approaches. By immunohistochemistry, AdipoR1 expression was ubiquitous in the rat brain . By contrast, AdipoR2 expression was more limited to specific brain areas such as hypothalamus, cortex and hippocampus. In arcuate and paraventricular hypothalamic nuclei, AdipoR1 and AdipoR2 were expressed by neurons and astrocytes. Furthermore, using transgenic green fluorescent protein (GFP) mice, we showed that AdipoR1 and AdipoR2 were present in POMC and NPY neurons in the arcuate nucleus. Finally, adiponectin treatment by intracerobroventricular injection induced AMPK phosphorylation in the rat hypothalamus. This was confirmed in vitro study using hypothalamic membrane fractions.

In conclusion, AdipoR1 and R2 are both expressed by neurons (including POMC and NPY neurons) and astrocytes in the rat hypothalamic nuclei. Adiponectin is able to increase AMPK phosphorylation in the rat hypothalamus. These data reinforced a potential role of adiponectin and its hypothalamic receptors in the control of energy homeostasis.

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