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This Article Accepted manuscript (PDF) All Versions of this Article: JOE-08-0237v1 199/3/425    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tanikawa, M. Articles by Okuda, K. Search for Related Content PubMed PubMed Citation Articles by Tanikawa, M. Articles by Okuda, K.

M Tanikawa, Graduate School of Natural Science and Technology, Laboratory of Reproductive Endocrinology, Okayama University, Okayama, Japan
H Lee, Graduate School of Natural Science and Technology, Laboratory of Reproductive Endocrinology, Okayama University, Okayama, Japan
K Watanabe, Division of Applied Life Science, Graduate School of Integrated Scince and Art, University of East Asia, Yamaguchi, Japan
M Majewska, Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
D Skarzynski, Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
D Lee, Division of Animal Resources, Collegeof Animal Resource Science, Kangwon National University, Chuncheon, Korea, Republic of
S Park, Animal Science, National Institute of Animal Science, Seonghwan 330-801, Korea, Republic of
C Park, Department of Animal Biotechnology, College of Animal Life Science, Kangwon National University, Chuncheon, Korea, Republic of
T Acosta, Graduate School of Natural Science and Technology, Laboratory of Reproductive Endocrinology, Okayama University, Okayama, 700-8530, Japan
K Okuda, Graduate School of Natural Science and Technology, Laboratory of Reproductive Endocrinology, Okayama University, Okayama, Japan

Correspondence: Tomas Acosta, Email: acosta{at}cc.okayama-u.ac.jp

Abstract

Interleukin-1 (IL-1) is a potent stimulator of prostaglandin production in bovine endometrium. The aims of the present study were to determine the cell types responsible for the secretion of prostaglandin (PG)E2 and PGF2 in response to IL-1, and the intracellular mechanisms of IL-1 action. Cultured bovine endometrial cells were exposed to IL-1 or IL-1β for 24 h. IL-1 and IL-1β stimulated PGE2 and PGF2 production in the stromal cells, but not in the epithelial cells. The stimulatory effect of IL-1 on PG production was greater than that of IL-1β. Stimulatory action of IL-1 on PG production was augmented by supplementing arachidonic acid (AA). When the stromal cells were incubated with IL-1 and inhibitors of phospolipase (PL) C or PLA2 (anthranilic acid; 1 µM), only PLA2 inhibitor completely stopped the stimulatory action of IL-1 on prostaglandin production. Moreover, a specific cyclooxygenase-2 (COX-2) inhibitor blocked the stimulatory effect of IL-1 on PG production. IL-1 (0.06 nM) promoted COX-2 and microsomal PGE synthase-1 (PGES1) gene and its protein expression. The expression of COX-1, PGES2, PGES3 and prostaglandin F synthase (PGFS) mRNA was not affected by IL-1 in the stromal cells. The overall results indicate that 1) the target of IL-1 and IL-1β for stimulating PG production is the stromal cell, 2) IL-1 is far more potent stimulator than IL-1β, 3) the stimulatory effect of IL-1 on PG production is mediated via the activation of PLA2 and COX-2, 4) IL-1 induced PG production by increasing expressions of COX-2 and PGES1 mRNAs and their proteins in bovine stromal cells.