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K Goosse, BANI, Universite Catholique de Louvain, Louvain-la-Neuve, Belgium
T Bouckenooghe, BANI, Universite Catholique de Louvain, Louvain-la-Neuve, Belgium
M Balteau, Louvain-la-Neuve, Belgium
B Reusens, Louvain-la-Neuve, Belgium
C Remacle, Louvain-la-Neuve, Belgium

Correspondence: Kevin Goosse, Email: k_goosse{at}hotmail.com

Abstract

An increased vulnerability of adult β-cell seems to be programmed in early life as adult islets from the progeny of dams fed a low protein diet exhibited an increased apoptotic rate after cytokines stimulation. This was prevented by maternal taurine supplementation. Here, we investigated the mechanisms implicated in such an increased vulnerability and how taurine exerts its protective role. Along gestation and lactation, Wistar rats were fed a 20% (Control [C group]) or an isocaloric 8% protein diet (Recovery [R group]) supplemented or not with taurine (CT and RT groups respectively). Offspring received a 20% protein diet after weaning. Islets from 3 month-old females were isolated and cultured for 48h before being incubated with or without cytokines for 24h. In unstimulated islets, apoptotic rate and NO. secretion were higher in R than in C. Both GADD153mRNA and protein were increased, whereas mRNA of mitochondrial gene ATPase6 was down-regulated in R group compared to C. In the RT group, taurine protected from apoptosis and restored a normal NO. production, GADD153 as well as ATPase6 mRNA expression. After cytokines-induction, apoptosis and NO. secretion were still increased in R compared to C but both parameters were normalized in RT group. In conclusion, maternal low protein diet programmes a different pattern of gene expression in islet-cells of adult progeny. Higher NO. production by these islets could be an important actor in the subsequent cell death. The prevention of these events by maternal taurine supplementation emphasises the importance of taurine during endocrine pancreas development.

This article has been cited by other articles:

				N. Theys, T. Bouckenooghe, M.-T. Ahn, C. Remacle, and B. Reusens Maternal low-protein diet alters pancreatic islet mitochondrial function in a sex-specific manner in the adult rat Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2009; 297(5): R1516 - R1525. [Abstract] [Full Text] [PDF]