Supplementary figures

Files in this Data Supplement:

• Supplementary figure 1 - Growth of Slc2a3+/+, Slc2a3+/- and Slc2a3-/- blastocyts in culture: Blastocyts of Slc2a3+/- intercrosses were flushed from the uteri at embryonic day 3.5 and cultured in vitro. Outgrowth of Slc2a3+/+, Slc2a3+/- and Slc2a3-/- blastocyts was documented after the indicated days in culture. Genotyping of blastocyts was performed by PCR as described in Material and Methods. TG, trophoblast giant cells; ICM, inner cellular mass.
• Supplementary figure 2 - Reduced proliferation in Slc2a3 mutant embryos: Serial sagittal sections of uteri from heterozygous matings at day 7.5 pc were stained with an antibody against Ki67 in order to detect proliferating cells. Some Ki67 positive cells in the ectoplacental cone and the embryonic endoderm are marked by arrowheads