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Journal of Endocrinology (2008) 198, 119-125       DOI: 10.1677/JOE-08-0125
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Low replacement doses of thyroxine during food restriction restores type 1 deiodinase activity in rats and promotes body protein loss

 Renata Lopes Araujo, Bruno Moulin de Andrade, Álvaro Souto Padron de Figueiredo, Monique Leandro da Silva, Michelle Porto Marassi, Valmara dos Santos Pereira, Eliete Bouskela1 and Denise P Carvalho

Laboratório de Fisiologia Endócrina do Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, CEP 21949-900, Brazil1 Laboratório de Pesquisas em Microcirculação, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, CEP 20550013 Brazil

(Correspondence should be addressed to D P Carvalho; Email: dencarv{at}biof.ufrj.br)


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Declaration of Interest
 References
 
During food restriction, decreased basal metabolic rate secondary to reduced serum thyroid hormones levels contributes to weight loss resistance. Thyroxine (T4) and 3,3',5-tri-iodothyronine (T3) administration during caloric restriction produce deleterious side effects; however, the administration of physiological doses of T4 during food restriction has never been evaluated. The aim of this study was to analyze the effects of low replacement doses of T4 in Wistar rats subjected to 40% food restriction. Food restriction for 30 days led to significantly reduced liver type 1 deiodinase activity, serum TSH, leptin, T4, T3, metabolic rate, and body mass. The significant reduction in hepatic deiodinase activity found during food restriction was normalized in a dose-dependent manner by T4 replacement, showing that decreased type 1 deiodinase (D1) activity is secondary to decreased serum thyroid hormone levels during caloric restriction. The lowest replacement dose of T4 did not normalize resting metabolic rate, but was able to potentiate the effects of food restriction on carcass fat loss and did not spare body protein. The highest dose of T4 produced a normalization of daily oxygen consumption and determined a significant reduction in both carcass fat and protein content. Our results show that serum T4 normalization during food restriction restores serum T3 and liver D1 activity, while body protein is not spared. Thus, decreased serum T4 during caloric restriction corresponds to a protective mechanism to avoid body protein loss, highlighting the importance of other strategies to reduce body mass without lean mass loss.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Declaration of Interest
 References
 
Moderate reduction in caloric intake has well-known systemic consequences, including weight loss and a decrease in fat mass; however, homeostatic mechanisms impair further weight loss after long periods of food restriction, such as the decrease in basal metabolic rate (Ravucin et al. 1985, Rosenbaum et al. 2002). The responses to food restriction have been well documented in a number of species, including humans (Kelley et al. 1993), monkeys (Kemnitz et al. 1994), rats (Dean et al. 1998), and mice (Harris et al. 1994). Thyroid hormones are the predominant regulators of basal metabolic rate and their serum levels are reduced during caloric deprivation (Vagenakis et al. 1977, Scriba et al. 1979, Kinlaw et al. 1985, Douyon & Schteingart 2002). Since thyroid hormones seem to play a crucial role in energy homeostasis by regulating both energy intake and expenditure, analogs of these hormones have long been considered to be potential drugs for controlling body mass (Oh & Kaplan 1994, Moreno et al. 2003, Lanni et al. 2005, Villicev et al. 2007).

Decreased thyroid function seems to be related to impaired thyrotropin-releasing hormone (TRH) and thyroid-stimulating hormone (TSH) secretion (Ahima 2000, Krotkiewski 2000), and to changes in the peripheral deiodination of thyroid hormones (Bianco et al. 2002). Three iodothyronine deiodinases have been identified and characterized in different tissues. Type 1 deiodinase (D1) is found in liver, kidney, and thyroid gland and catalyzes both the outer and inner ring deiodination of iodothyronines, while types 2 (D2) and 3 (D3) deiodinases act exclusively as outer and inner ring deiodinases respectively (Bianco et al. 2002). Previous studies have shown that liver and pituitary D1 activities are significantly decreased during fasting in rats. However, since the main positive regulator of liver and kidney D1 activity is T3 (Bianco et al. 2002, Aceves et al. 2003), which is also reduced under caloric restriction, it is difficult to establish whether decreased D1 activity is related to a direct effect of fasting, per se, or secondary to serum thyroid hormone reduction (St Germain & Galton 1985, O'Mara et al. 1993, Aceves et al. 2003).

Although it has long been recognized that thyroid hormones modify body mass composition, these previous studies have used high T4 doses (25–250 µg/100 g body mass; Aranda et al. 1972, Okajima & Ui 1979, Müller & Seitz 1980), which lead to thyrotoxicosis and several deleterious effects on the organism such as protein loss, heart hypertrophy, and arrhythmias (Klein & Ojamaa 2001). However, the replacement of a low physiological dose of T4 during food restriction in normal rats has not been reported so far. The lowest T4 dose found in the literature has been tested by Burini et al. (1981) in a previous report using thyroidectomized rats under food restriction (2 µg T4/100 g b.w.), but apart from using thyroidectomized rats, no oxygen consumption or deiodinase activities have been evaluated. Some other previous reports describe the effects of T3 administration on food-restricted (FR) obese patients, and the great majority uses a high T3 dose (Bray et al. 1973, Wilson & Lamberts 1981). On the other hand, the effects of low replacement doses of T4 during a period of food restriction have not been studied so far, although an early report of leptin replacement and serum T4 restoration indicates beneficial actions in humans under caloric restriction (Rosenbaum et al. 2002).

In the present paper, our aim was to restore serum T4 levels during food restriction in male Wistar rats in order to determine whether physiological T4 replacement restores liver and kidney type 1 deiodinase activities, resting metabolic rate (RMR), and body mass composition.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Declaration of Interest
 References
 
Animals

Adult male Wistar rats were housed at controlled temperature (23 °C) with daily exposure to a 12 h light:12 h darkness cycle and free access to water and standard rat chow. This investigation conforms to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No.85-23, revised 1996) and was approved by the institutional animal welfare committee. All animals were individually housed for a 1-week acclimation period and baseline control food intake was assessed.

30 days food restriction

Food intake was assessed over 7 days for each rat by offering food ad libitum and measuring the quantity consumed. After this period, the ad libitum (control – C) group had free access to food, and the FR group received 60% of their individual baseline intake for 30 days, so that the food was 40% restricted. During the food restriction period, all rats were weighed every 2 days.

T4 treatment

After 15 days of the beginning of food restriction, control (C) and FR rats were randomly assigned to start chronic treatment by daily single s.c. injections of (L-T4 Sigma) or saline (0.9% NaCl). The use of T4 instead of T3 is of physiological relevance, since T4 is the major product of the thyroid gland and its secretion is decreased during food restriction. T4 treatment protocol was done as follows: 1.0 µg/100 g b.w. (FR T4 (1)), which has been used to restore the euthyroid status in hypothyroid animals (Ortiga-Carvalho et al. 1996), and a dose of 5.0 µg/100 g b.w. (FR T4 (5)). T4 administration was conducted for the last 15 days of experiment, every 1000 h for all groups of animals. The animals were killed 24 h after the last T4 administration by decapitation, and blood was collected from the trunk. Serum was obtained after centrifugation of blood at 1000 g for 20 min and stored at –20 °C until specific RIA (TSH, T3, T4 and leptin). Liver and kidney tissue samples were dissected out and stored at –70 °C until processing for D1 activity.

Body composition

Body composition (fat and protein) was determined by carcass analysis, as previously described (Toste et al. 2006). The retroperitoneal and epididymal fats were completely removed, weighed for evaluation of central adiposity and discarded. The total carcass protein concentrations were determined by the method of Lowry et al. (1951). The carcass results were expressed as g/100 g carcass. The retroperitoneal and epididymal fats were weighted and expressed as g/100 g b.w.

Resting metabolic rate

RMR was measured using open-circuit indirect calorimetry during 24 h, after 30 days of experimental time. The rats were individually placed in a respiration chamber (25x25x15 cm), air flow was maintained constant at 500 ml/min by a mass flow controller (Sable System International, Las Vegas, NV, USA). Oxygen was measured using an O2 analyzer (Sable System International). Oxygen consumption was recorded at 15 min intervals and the results were expressed in LO2/kg per h. To avoid disruption caused by an adaptation to the chamber, the first 6 h were excluded from the analyses.

Serum TSH, leptin, and total T3 and T4

Serum TSH levels were evaluated by a specific RIA obtained from the National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK, Bethesda, MD, USA), and expressed in terms of the reference preparation 2 (RP-2). Intra- and inter-assay coefficients of variation were 7.7 and 6.5% respectively and the sensitivity was 0.50 ng/ml.

Serum total T3 and T4 concentrations were measured using commercial RIA kits (T3: DSL – 3100 Active, sensitivity of 4.3 ng/dl, inter- and intra-assay coefficients of variation varied from 4.2 to 6.0 and 5 to 6.5% respectively; T4: DSL – 3200 Active, sensitivity of 0.4 µg/dl, inter- and intra-assay coefficients of variation varied from 7.1 to 7.4 and 2.9 to 5.1% respectively; DSL, TX, USA), based on the presence of specific antibodies adhered to the internal surface of propylene tubes. Rat hormone-stripped serum was used for standard curves of total T4, T3, and TSH.

Serum leptin concentrations were measured using a specific RIA for rat leptin obtained from the Linco Research Company (St Charles, MO 63304, USA). The kit uses 125I-labeled rat leptin and a rat leptin antiserum to determine the level of serum leptin by the double antibody/polyethylene glycol (PEG) technique. Intra- and inter-assay coefficients of variation were 3.3–6.8 and 4.1–2.0% respectively and the sensitivity was 0.50 ng/ml using 100 µl sample size.

All the procedures were carried out following the recommendations as given in the kits.

Deiodinase analyses

Type 1 iodothyronine deiodinase activity was evaluated as previously described by our group (Fortunato et al. 2006) and based on Berry et al. (1991). In short, tissue (liver or kidney) samples of 25 mg were homogenized in 1 ml of 0.1 M sodium phosphate buffer containing 1 mM EDTA, 0.25 M sucrose, and 10 mM dithiothreitol (pH 6.9). Homogenates (30 µg protein from liver or kidney) were incubated in a water bath in duplicate for 1 h at 37 °C with 1 µM rT3 (Sigma), equal volumes of 125I rT3 (Perkin–Elmer Life Sciences, Boston, MA, USA) previously purified using sephadex LH-20, and 10 mM dithiothreitol in 100 mM potassium phosphate buffer containing 1 mM EDTA (pH 6.9) in a reaction volume of 300 µl. Blank incubations were carried out in the absence of protein. The reaction was stopped after incubation at 4 °C followed by the addition of 100 µl fetal bovine serum (Cultilab, Campinas, Brazil) and 200 µl trichloroacetic acid (50%, v/v). The samples were centrifuged at 8000 g for 3 min, and the supernatant was collected for measurement of 125I liberated during the deiodination reaction.

Protein concentration in the homogenates was measured by the Bradford method (Bradford 1976), after incubation of homogenates with NaOH (2.5 M). The specific enzyme activity was expressed as picomoles of rT3 deiodinated/min/mg protein.

Statistical analyses

Results were expressed as mean±S.E.M. Body mass, fat and protein content, total serum T3, T4, TSH and leptin concentrations, deiodinase activity, and oxygen consumption were analyzed by two-way ANOVA, followed by Bonferroni multiple comparison tests. Statistical analyses were done using the software Graphpad Prism (version 4, Graphpad Software, Inc., San Diego, CA, USA). The differences were considered significant when P<0.05.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Declaration of Interest
 References
 
Body composition – mass, fat, and protein contents

Final body mass was lower in FR compared with C groups (Table 1, P<0.001), and the two doses of T4 did not produce any further body mass change (Table 1).


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  		Table 1 Body weight and composition of control (C) and food-restricted (FR) rats with or without thyroxine (T4) replacement. Values are expressed as mean±S.E.M. of at least ten animals per group from three to five experiments

 
Retroperitoneal and epididymal fat mass were significantly lower in FR compared with C groups (Table 1, P<0.001), and no further reduction in these fat compartments were observed with both T4 doses (Table 1). On the other hand, carcass fat was not affected by FR per se, while the two doses of T4 significantly decreased carcass fat mass (P<0.01). These results show that T4 effects on adipose tissue depends on the fat compartment analyzed.

Carcass protein content was not significantly different between C and FR groups, demonstrating that 40% food deprivation for 30 days did not affect body protein content. However, exogenous T4 administration during food restriction produced a significant decrease in body protein content with the use of either the lowest (FR T4 (1)) or the highest (FR T4 (5)) T4 doses (Table 1, P<0.001). We have also observed a significant decrease in carcass protein content in control rats fed ad libitum and treated with the highest dose of T4 (Table 1).

Serum TSH, leptin, and total T3 and T4

Serum TSH and leptin concentrations were significantly reduced (P<0.001) after 30 days of food restriction, and remained low in the T4 replaced groups (FR T4 (1) and (5); Table 2).


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  		Table 2 Serum thyroid-stimulating hormone (TSH), leptin, total tri-iodothyronine (T3) and thyroxine (T4) of control (C) and food-restricted (FR) Wistar rats with or without T4 replacement. Values are expressed as mean±S.E.M. of at least ten animals per group from three to five experiments

 
Serum T4 and T3 concentrations were significantly reduced after 30 days of food restriction (Table 2), and increased in FR rats treated with the lowest dose of T4 (Table 2). Serum T4 levels significantly increased in FR T4 (5) in relation to FR T4 (1) and FR (Table 2); however, serum T3 did not increase significantly in the FR T4 (5) (Table 2).

Resting metabolic rate

Figure 1 summarizes the results collected over a 24-h period of time. The results were plotted as mean oxygen consumption and were analyzed as the area under the curve. There was a 45% reduction in total oxygen consumption when FR was compared with the control group (Fig. 1, P<0.05). The lowest dose of T4 did not normalize RMR, but treatment with the highest T4 dose restored RMR to control values, suggesting that the reduction in basal metabolism observed during food deprivation is, in part, dependent on the decreased serum thyroid hormone concentrations.


Figure 1
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  		Figure 1 Resting metabolic rate in control (C) and food-restricted (FR) Wistar rats with saline (dark gray bar) or T4 replacement (1 µg/100 g b.w., gray bar and 5 µg/100 g b.w., light gray bar). (A) Oxygen consumption during 18 h (C; n=20 FR; n=7 FR 1; n=4 FR 5; n=5) and (B) Area under the curve. *P<0.05 versus control.

 
Deiodinase activity

Liver type 1 iodothyronine deiodinase activity was significantly reduced after 30 days of food restriction (Fig. 2A, P<0.05). When the groups submitted to food restriction were treated with exogenous T4, D1 activity increased in a dose-dependent manner (Fig. 2A, P<0.001).


Figure 2
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  		Figure 2 Type 1 iodothyronine deiodinase (D1) activity in control (C) and 40% food-restricted (FR) rats with saline (white bar) or T4 replacement (1 µg/100 g b.w, light gray bar and 5 µg/100 g b.w, dark gray bar). (A) D1 Hepatic (C, n=8; FR, n=13; FR T4 (1), n=12; FR T4 (5), n=15) and (B) D1 Kidney (C, n=9; FR, n=15; FR T4 (1), n=12; FR T4 (5), n=13). Results are shown as mean±S.E.M. *P<0.05 versus control; **P<0. 01 versus control and FR; ***P<0.001 versus all the other groups.

 
Kidney D1 activity was not significantly different after 30 days of food restriction (Fig. 2B). Treatment with the lowest dose of T4 in FR rats did not change the enzyme activity and only the highest dose of T4 led to a significant increase in kidney D1 activity when compared with the FR group (Fig. 2B, P<0.01).


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Declaration of Interest
 References
 
We show herein that 30 days of food restriction leads to reduction in serum TSH, T4, T3, and leptin, which parallel the reduction in body mass, retroperitoneal, and epididymal fat compartments; however, no changes were observed on carcass fat and protein contents. Interestingly, the replacement of T4 to FR groups with a dose just able to normalize serum T4 did not affect body mass and retroperitoneal and epididymal fats, although significant body protein and fat carcass loss were detected. These results differ from studies that described significant modifications in body mass when animals and humans were treated with higher doses of T4 or tri-iodothyronine (25–250 µg/100 g body mass). Bray et al. (1973) evaluated the effect of tri-iodothyronine (150 µg/day) in combination with a liquid formula diet (900 kcal/day) and showed that T3 produces a higher body weight loss. Wilson & Lamberts (1981) also showed a significant reduction on body mass using T3 (25 µg/day) in obese patients during drastic calorie restriction. These differences could be related to the fact that in these studies a large dose of T3 was used, while we used a low dose of T4. Our results also show the concomitant reduction of serum T4 and RMR during food restriction. Also, Moreno et al. (2003) showed that a single dose of T3 (25 µg/day) to fasted rats restores uncoupling protein-3, uncoupling by regulating the levels of coenzyme Q. The changes in metabolism created by the association of two inverse energy conditions: food deprivation (decreased energy) and T4 administration (increased energy) led to a significant decrease in body protein compartment, showing that the reduction in RMR during FR could represent a mechanism of physiological protection against body protein loss, as previously suggested (Gardner et al. 1979).

The effects of food restriction on serum TSH, leptin, and thyroid hormones were strikingly similar to those previously described by other authors (Salih et al. 1993, Gazdag et al. 1999, Davidson et al. 2002). The mechanisms that explain T4 reduction during calorific restriction have been partially defined. Serum leptin decreases together with increased serum corticosterone during fasting, what leads to reduced pro-TRH in the hypothalamic paraventricular nucleus, with a consequent decrease of serum TSH (Legradi et al. 1997, Ahima et al. 2000, Coppola et al. 2005). However, decreased D1 activity could also contribute to the lower serum T3 found during caloric deprivation. It has not been established to what extent the peripheral reduction of T3 production could also be relevant for the decreased RMR and increased resistance to further weight loss. Conjugation of thyroid hormones involves glucuronidation or sulfation of the phenolic hydroxyl group, which increases its water solubility and facilitates urinary and biliary clearance (De Herder et al. 1988, Burchell & Coughtrie 1989). Sulfation also promotes the inactivation of thyroid hormones, because the inner ring deiodination of sulfated T4 and T3 by D1 is accelerated 40- to 200-fold, whereas the outer ring deiodination of sulfated T4 is completely blocked (Visser 1994). This mechanism might be implicated in the drop of serum T3 levels by as much as 33% in subjects submitted to weight loss programs (Rosenbaum et al. 2000).

During FR, the use of both T4 doses increased serum T4 concentrations in a dose-dependent manner. However, serum T3 did not increase accordingly, despite the fact that type 1 deiodinase activity was increased. Considering the fact that T4 might be deiodinated in the outer or inner rings, sulfated or glucuronidated in the liver, the respective products to be formed are: T3, reverse T3, sulfated T3 or sulfated rT3, and others. During food restriction T4 sulfation seems to increase. Maglich et al. (2004), have shown that during energy deprivation the main product of type 1 deiodinase is not T3 as the outer ring deiodination of sulfated T4 is almost undetectable, whereas the rate of inner ring deiodination increases over 130-fold increasing the production of sulfated rT3. Recently, Emi et al. (2007) have demonstrated that the transcription and activity of rat uridine diphosphate-glucuronosyltransferase 1A7 is positively regulated by T4, and the enzyme in turn metabolizes and inactivates T4. To date, the regulation of uridine diphosphate-glucuronosyltransferase 1A7 during food restriction has not been determined, but it is tempting to speculate whether under calorific restriction it could be more sensitive to serum T4 variations. Thus, injected T4 could be converted into metabolites other than T3 during food restriction, as the same dose of T4 administered to normal rats had indeed increased serum T3 as expected, different from what was found in FR animals. We can only hypothesize that during calorific restriction, liver metabolization of T4 and T3 is largely regulated and might be responsible for the lower serum T3 due to increased clearance of these hormones.

Our findings show that decreased liver D1 activity is a consequence rather than the cause of decreased serum T3 during food restriction. Moreover, normalization of serum T4 and T3 in FR rats led to a significant decrease in protein and carcass fat contents, showing unequivocally the deleterious effects of any strategy to normalize these hormones during calorie deprivation with the aim of body weight control.

We conclude that decreased serum T4 during calorie restriction is a protective mechanism to avoid body protein loss. Other strategies such as the use of thyroid hormone analogs that might spare body protein are of great importance.


   Declaration of Interest
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Declaration of Interest
References
 
The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.


   Funding
 
This work was supported by grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Programa de Núcleos de Excelência (PRONEX/FAPERJ), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).


   Acknowledgements
 
We are grateful for the technical assistance of Norma Lima de Araújo Faria, Advaldo Nunes Bezerra, and Wagner Nunes Bezerra. Renata Lopes Araujo and Norma Lima de Araújo Faria were recipients of fellowships from CNPq during the present study.


   References
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 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Declaration of Interest
 References
 
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Received in final form 14 April 2008
Accepted 21 April 2008
Made available online as an Accepted Preprint 21 April 2008




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