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Unité de Physiologie de la Reproduction et des Comportements and
1 Station de Recherches Avicoles, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
2 INSERM UMR S671, Centre Biomédical des Cordeliers, Université Pierre et Marie Curie-Paris 6, Paris F-75006, France
(Requests for offprints should be addressed to J Dupont; Email: jdupont{at}tours.inra.fr)
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Abstract |
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1/2, ß1/2, and 
1/2) and proteins (1/2 and ß1/2) can be found in the hen preovulatory follicles and precisely in both granulosa and theca cells. These preovulatory follicles are organized in a hierarchy according to their size (F5/6 to F1). The smallest number (F1) corresponds to the largest size and the latest mature stage. Phosphorylation of AMPK on Thr172 and of acetyl-CoA carboxylase on Ser79 are higher in F4 and F3 than in F1 granulosa cells. However, they are not affected in F4–F1 theca cells. Treatment with 1 mM 5-amino-imidazole-4-carboxyamide-1-ß-D-ribofuranoside (AICAR), an activator of AMPK, dose dependently increased phosphorylation of AMPK on Thr172 in primary F3/4 and F1 granulosa cells. In the absence of FSH, AICAR treatment increased progesterone, P450 side chain cleavage and steroidogenic acute regulatory (StAR) production in both F3/4 and F1 granulosa cells. However, in the presence of FSH, AICAR treatment for 36 h increased progesterone secretion, StAR protein levels and reduced extracellular signal-regulated kinase (ERK)1/2 phosphorylation in F3/4 granulosa cells. Opposite data were observed in F1 granulosa cells. Adenovirus-mediated expression of dominant-negative AMPK totally restored the effects of AICAR on FSH-induced progesterone secretion, StAR protein production, and ERK1/2 phosphorylation in F3/4 and F1 granulosa cells. Using a specific inhibitor of ERK1/2 (U0126), we also showed that this kinase is a negative regulator of the FSH-induced progesterone secretion in F3/4 and F1 granulosa cells, suggesting that AICAR-mediated AMPK activation modifies FSH-induced progesterone secretion differently through the ERK1/2 signaling pathway in hen F3/4 and F1 granulosa cells. |
Introduction |
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AMPK is involved in the regulation of cellular energy homeostasis; it regulates the expression and activity of enzymes involved in the synthesis of fatty acid, cholesterol, and glycogen (Carling et al. 1987). Recent evidence suggests that AMPK is also a link between the sensing of intracellular energy levels and the regulation of protein synthesis (Bolster et al. 2002). AMPK is activated by some conditions that deplete cellular ATP (Corton et al. 1994). Thus, under extreme nutritional conditions, AMPK responds to increases in the AMP/ATP ratio by switching off ATP-consuming pathways and switching on pathways for ATP generation (Unger 2004). AMPK is a heterotrimer comprising one -catalytic subunit, one ß-regulatory subunit and one -regulatory subunit (Woods et al. 1996, Stapleton et al. 1997). In mammals, there are multiple isoforms of each subunit encoded by separate genes (1, 2, ß1, ß2, 1, 2, and 3) (Hardie 2003, Carling 2004). AMPK activity depends upon phosphorylation of the Thr172 residue in the activation loop of the -subunit by an upstream kinase named LKB1, (Hawley et al. 1996) and both phosphorylation and dephosphorylation processes are sensitive to AMP (Davies et al. 1995). The adenosine analog, 5-amino-imidazole-4-carboxyamide-1-ß-D-ribofuranoside (AICAR), has been largely used as an activator of the AMPK system (Corton et al. 1995). In mammals, the metabolic effects of AICAR have been studied extensively in skeletal muscle, adipose tissue, and liver. Recently, the presence of AMPK and the effects of AICAR have been investigated in rodent ovary, both in oocytes (Downs et al. 2002) and granulosa cells (Tosca et al. 2005). In mouse oocytes, AMPK may play a key role in meiotic induction (Downs et al. 2002, Larosa & Downs 2006). In rat granulosa cells, we have demonstrated that AMPK activation reduces basal and FSH- and/or IGF-I-induced progesterone secretion (Tosca et al. 2005). The AMPK system has been investigated very recently in chicken. Chicken LKB1 and AMPK subunits have been totally or partially sequenced showing a very high homology at the amino acid level compared with their human homologs (Proszkowiec-Weglarz et al. 2006). AMPK subunits are expressed in various chicken tissues (Proszkowiec-Weglarz et al. 2006); however, chicken ovary was not included in this study.
The objectives of the present work were to identify and characterize the expression and activity of AMPK in vivo in hen preovulatory follicles during their development. We also examined the potential role of AMPK in hen granulosa differentiation in response to FSH by using AICAR and an adenovirus mediated expression of dominant-negative AMPK in cultured F3/4 and F1 granulosa cells.
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Materials and Methods |
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Purified ovine FSH-20 (oFSH) (lot no. AFP-7028D, 4453 IU/mg, FSH activity = 175 times activity of oFSH-S1) was used for culture treatment (a gift from NIDDK, National Hormone Pituitary Program, Bethesda, MD, USA). Dulbecco’s modified Eagle’s medium (DMEM), penicillin, and streptomycin were purchased from Invitrogen, and methyl [3H] thymidine from Perkin Elmer Life and Technological Sciences (Boston, MA, USA). AICAR was obtained from Sigma.
Antibodies
Rabbit polyclonal antibodies to phospho-extracellular signal-regulated kinase (ERK)1/2 (Thr202/Tyr204), phospho-AMPK Thr172, and acetyl-CoA carboxylase (ACC) were purchased from New England Biolabs, Inc. (Beverly, MA, USA). Rabbit polyclonal antibodies to ERK2 (C14) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal anti-actin antibodies were obtained from Oncogene Research (Boston, MA, USA). Rabbit polyclonal antibodies to AMPK1/2 (-pan AMPK antibody recognizes AMPK1 and 2 at 63 kDa), AMPKß (recognizes AMPKß1 (40 kDa) and ß2 (34 kDa)) and phospho-ACC (pACC) Ser79 were obtained from Upstate Biotechnology, Inc. (Lake Placid, NY, USA). Monoclonal antibody to green fluorescent protein (GFP) was obtained from Clontech. Rabbit polyclonal antibodies to P450 side chain cleavage (P450scc), steroidogenic acute regulatory (StAR) protein, and 3ß-hydroxysteroid dehydrogenase (HSD3ß) were generously provided by Dr Dale Buchanan Hales (University of Illinois, Chicago, IL, USA) and Dr Van Luu-The (CHUL Research Center and Laval University, Quebec City, Quebec, Canada) respectively. All antibodies were used at 1:1000 dilution in Western blotting.
Animals, granulosa cells isolation and culture
Laying breed hens (50–60 weeks old) (ISA Brown, egg layer type; Institut de Selection Animale, Saint Brieuc, France) were housed individually in laying batteries with free access to feed and water and were exposed to a 15 h light:9 h darkness photoperiod, with lights-on at midnight. Individual lay patterns were monitored daily. The average clutch size was about 12 eggs. Hens were sacrificed by an overdose of pentobarbital (Sanofi-santé, Libourne, France) between 10 and 12 h before next oviposition and the ovary was immediately removed and placed in ice-cold sterile 1% NaCl saline solution for immediate use. This pentobarbital treatment did not have any significant effect on basal levels of AMPK phosphorylation. All procedures described herein were approved by the Agricultural Agency and the Scientific Research Agency and conducted in accordance with the guidelines for Care and Use of Agricultural Animals in Agricultural Research and Teaching.
Granulosa cells from F1 and F3/4 follicles were dispersed in 0·3% collagenase type A (Roche) in F12 medium containing 5% fetal bovine serum (FBS). Cells were recovered by centrifugation, washed with fresh medium, and counted in a hemocytometer. The viability of F3/4 or F1 granulosa cells estimated by Trypan Blue exclusion was about 95%. The culture medium was DMEM supplemented with 100 U/ml penicillin, 100 mg/l streptomycin, 3 mmol/l L-glutamine and 5% FBS. The cells were initially cultured for 24 h with no treatment and incubated in fresh culture medium with or without test reagents for the appropriate time. All cultures were maintained under a water-saturated atmosphere of 95% air/5% CO2 at 37 °C.
RNA isolation and reverse transcriptase (RT)-PCR
Total RNA was extracted from tissues (ovary and pectoralis muscle) and fresh cells (theca and granulosa cells removed from different preovulatory follicles, F5/6 to F1) using Trizol reagent, according to the manufacturer’s procedure (Invitrogen). RNA samples were quantified by measuring the absorbance at 260 nm and treated with DNase to remove any possible DNA contamination. Samples were stored at –80 °C until use. RT-PCR was performed to test the expression of different ampk subunits as previously described (Tosca et al. 2005). Specific sets of primer pairs designed to amplify parts of the different subunits of ampk are shown in Table 1
. PCR amplifications without RNA or without reverse transcriptase were performed in parallel as negative controls. RT-PCR disposables were purchased from Sigma, except Moloney murine leukemia virus reverse transcriptase and RNase inhibitor from Promega. All the PCR products were cloned into pCRII-TOPO vector (TOPO TA Cloning kit, Invitrogen), and then sequenced.
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Lysates of granulosa and theca cells or ovary were solubilized and centrifuged as previously described (Tosca et al. 2005). Cell extracts were then submitted to electrophoresis on 10% (w/v) SDS-PAGE under reducing conditions and electrotransferred as previously described (Tosca et al. 2005). The membranes were then incubated overnight at 4 °C with appropriate antibodies (final dilution 1:1000) in Tris-buffered saline (TBS, 2 mM Tris–HCl, pH 8, 15 mM NaCl, pH 7·6) containing 0·1% Tween-20 and 5% nonfat dry milk powder (NFDMP). After washing in TBS–Tween-20 0·1%, nitrocellulose membranes were incubated for 2 h at room temperature with a horseradish peroxidase-conjugated anti-rabbit or anti-mouse immunoglobulinG (final dilution 1:10 000; Diagnostic Pasteur, Marnes-la-Coquette, France) in TBS–0·1% Tween–20 NFDMP 5%. After washing in TBS–Tween-20 0·1%, the signal was detected by enhanced chemiluminescence (Amersham). The films were analyzed and signals quantified with MacBas V2·52 software (Fuji PhotoFilm, Inc., New Haven, CT, USA).
Thymidine incorporation into granulosa cells
Granulosa cells were seeded in 24-well dishes (2 x 105 viable cells per 500 µl). After 48 h, cells were serum starved overnight and [3H]thymidine (1·5 µCi/ml) was added in the absence or presence of AICAR (1 mM), IGF-I (10–8 M) and FSH (10–8 M). After 24 h, excess [3H]thymidine was removed by washing twice with PBS; the cells were fixed with ice-cold trichloroacetic acid (50%) and lysed by NaOH (0·5 M). The radioactivity in the cells was determined after resuspension by scintillation counting in a ß-photomultiplier. Furthermore, the protein concentration was determined using a colorimetric assay (Kit BC assay, Uptima Interchim, Montluçon, France) to normalize the thymidine incorporation data.
Adenoviruses and infection of hen granulosa cells
Dominant-negative AMPK adenovirus (1-DN) was constructed from AMPK1 carrying the Asp157 to Ala (D157A) mutation as described previously (Woods et al. 2000). Recombinant adenovirus (Ad) was propagated in HEK293 cells, purified by cesium chloride density centrifugation, and stored as described previously (Woods et al. 2000). Hen granulosa cells from preovulatory follicles (F3/4 and F1) were infected with 10 or 100 pfu/cell adenovirus in serum-starved DMEM for 24 h and cultured for another 24 h in the presence or absence of FSH and 1 mM AICAR. Preliminary studies revealed that within 24 h of infection (100 pfu/cell) with a GFP-expressing virus, the majority of granulosa cells (>75%) expressed GFP.
Progesterone RIA
The concentration of progesterone (P4) in the culture medium of granulosa cells was measured after 24 or 36 h of culture as indicated in the legend of the figures by an RIA protocol as previously described (Tosca et al. 2005) and adapted to measure steroids in cell culture media. The limit of detection of P4 was 12 pg/tube (60 pg/well) and the intra-and interassay coefficients of variation were less than 10 and 11% respectively. Results were expressed as the amount of steroids secreted for 24 or 36 h (as indicated in the legends of the figures) per 100 µg protein.
Statistical analysis
All experimental data are presented as means ± S.E.M. One-way ANOVA was used to test differences. If ANOVA revealed significant effects, the means were compared by Newman’s test, with P < 0·05 considered significant. Different letters indicate significant differences.
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Results |
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-phosphorylation in hen preovulatory follicles
RT-PCR analysis was first performed on total RNA from hen ovary. This resulted in the amplification of different cDNAs corresponding to specific fragments of ampk 1, ampk 2, ampk ß1, ampk ß2, ampk 1, and ampk 2. As shown in Fig. 1a, all these ampk subunits were expressed in both theca and granulosa cells from follicles at different preovulatory stages (F5/6 to F1). In contrast, ampk 3 subunit mRNA was undetectable in ovary but present in muscle. By Western blot, AMPK1/2 (62 kDa), AMPKß1 (40 kDa), and ß2 (34 kDa) proteins were shown to be present in whole hen ovary and precisely in theca and granulosa cells from different preovulatory follicles (Fig. 1b). Furthermore, quantitative analysis indicated no significant difference in the protein level of AMPK1/2, AMPKß1, and AMPKß2 in granulosa and theca cells along with the various preovulatory follicle categories. However, there is a threefold decrease in the phosphorylation level of the -subunit of AMPK on the Thr172 residue in granulosa cells when comparing F1 follicle to F3 or F4 follicles (Fig. 1c, left panel). The phosphorylation of one of the downstream targets of AMPK, ACC on the Ser79 residue follows a similar pattern (Fig. 1c, middle panel). Interestingly, no difference in the levels of AMPK Thr172 (Fig. 1c, right panel) and ACC Ser79 phosphorylation (data not shown) was detected in theca cells from F4 to F1 preovulatory follicles. Thus, AMPK is present and active in vivo in granulosa and theca cells from preovulatory follicles. Moreover, AMPK Thr172 phosphorylation decreases in only granulosa cells during the preovulatory follicle development.
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We next determined whether AMPK can be activated by AICAR in vitro in primary F3/4 and F1 granulosa cells. As shown in Fig. 2, the treatment of granulosa cells from preovulatory follicle F1 elicited a Thr172 phosphorylation of AMPK in a dose-dependent manner. After a 36 h exposure to AICAR, AMPK was phosphorylated maximally (about fourfold) for a 1 mM concentration. Unless otherwise indicated, this concentration will be used in subsequent studies to determine the biological effects of AICAR in granulosa cells. Furthermore, we have observed that AICAR also increased phosphorylation of ACC at Ser79 in a dose-dependent manner, paralleling the stimulation of Thr172 phosphorylation of AMPK (Fig. 2). Similar results were observed in granulosa cells from F3/4 preovulatory follicles (data not shown). Thus, AMPK is active in cultured granulosa cells from preovulatory hen follicles.
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We next investigated the effect of AICAR treatment on progesterone production in F3/4 and F1 granulosa cells. As expected (Etches & Duke 1984), in the absence of FSH, progesterone production was lower in F3/4 (1·5 ± 0·2 ng/ml per 100 µg protein) than in F1 granulosa cells (39 ± 2 ng/ml per 100 µg protein; Fig. 3a and b). However, FSH-induced progesterone secretion was higher in F3/4 than in F1 (Fig. 3a and b). Indeed, FSH increased by about 20-fold progesterone secretion in F3/4, whereas it increased it by only 1·5-fold in F1 granulosa cells. In the absence of FSH, a 36-h AICAR treatment increased progesterone production by about twofold in F3/4 granulosa cells and by 1·5-fold in F1 granulosa cells (P < 0·05). In the presence of FSH, AICAR treatment increased progesterone secretion by twofold in F3/4 granulosa cells (Fig. 3a). However, AICAR unexpectedly reduced FSH-induced progesterone secretion by about 30% as compared with the FSH treatment alone in F1 granulosa cells (Fig. 3b).
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). Interestingly, AICAR treatment in the presence of FSH increased by about threefold, the content in StAR protein in F3/4 granulosa cells, whereas it decreased by twofold those produced in F1 granulosa as compared with FSH alone (Fig. 3c and d). AICAR treatment increased by almost twofold FSH-induced P450scc production in F1 granulosa cells (Fig. 3d), whereas it had no effect in F3/4 granulosa cells (Fig. 3c). AICAR did not modify the FSH-induced HSD3ß protein level in both F3/4 and F1 granulosa cells. Thus, AICAR treatment has parallel effects on progesterone and StAR expressions, but intriguingly, the direction of these effects is opposite when considering F3/4 and F1 granulosa cells.
Overexpression of a dominant-negative AMPK opposes AICAR effects on FSH-induced progesterone production and STAR protein level in F3/4 and F1 granulosa cells
Although AICAR is widely used as a pharmacological activator of AMPK, AICAR has many different cellular effects (Corton et al. 1995, Kemp et al. 1999). Therefore, we tested whether the differential effects of AICAR on the response to FSH observed between F3/4 and F1 granulosa cells for progesterone and StAR production were indeed mediated by AMPK. F3/4 and F1 granulosa cells were infected for 24 h with a dominant-negative (1-DN) form of AMPK or GFP as a control using Ad vectors. 1-DN and GFP proteins were detected in granulosa cells infected with Ad 1-DN or Ad GFP (10 and 100 pfu/cell) for 24 h (data not shown). Furthermore, 1-DN (100 pfu) significantly attenuated basal AMPK Thr172 phosphorylation in both F1 and F3/4 granulosa cells (data not shown). Infection of granulosa cells with Ad GFP had no effect on AMPK1/2 expression or AMPK Thr172 phosphorylation. Expression of 1-DN abolished AICAR-induced increase in the production of both progesterone and StAR protein in response to FSH (24 h) in F3/4 granulosa cells (Fig. 4a). Furthermore, it also abolished AICAR-induced decrease in the production of both progesterone and StAR protein in response to FSH (24 h) in F1 granulosa cells (Fig. 4b). Thus, these data demonstrate that AMPK is involved in the AICAR effects (Fig. 4a and b). Furthermore, we did not observe any significant effect on progesterone production and StAR protein level in response to the 1-DN treatment alone or in combination with FSH (data not shown). Infection of cells with a control GFP virus for 24 h had no effect (data not shown). As revealed by the Trypan Blue staining assay, we did not detect any difference in the viability between cells not infected and cells infected with 100 pfu 1-DN or GFP (data not shown).
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We also investigated whether the dose of AICAR used affected the number and/or the viability of F3/4 and F1 granulosa cells in culture. [3H]thymidine incorporation by granulosa cells treated with 1 mM AICAR was tested after 24 h in culture in the presence or in the absence of FSH or IGF-I (as a positive control). FSH treatment did not affect the level of [3H]thymidine incorporation, whereas IGF-I treatment used as a positive control did (data not shown). Furthermore, AICAR treatment had no effect either in the absence or in the presence of FSH in both F1 and F3/4 granulosa cells (data not shown). As revealed by the Trypan Blue staining assay, we did not detect any difference in the viability of cells between controls and cells treated with 1 mM AICAR for 36 h (data not shown). Thus, AICAR treatment (1 mM) for 36 h did not affect cell number or viability in F3/4 and F1 granulosa cells.
Role of the ERK1/2 MAPK in the differential effects of AICAR mediated by AMPK on FSH-induced progesterone production in F3/4 and F1 granulosa cells
It has been shown that AICAR can modulate the ERK1/2 signaling pathways in different cell types (Kim et al. 2001, Chen et al. 2002). In the hen, it has been established that this latter signaling pathway can negatively regulate FSH-induced granulosa cell differentiation in vitro (Johnson et al. 2001, 2002, Woods et al. 2005). Thus, we first determined the ability of AICAR treatment to modulate the activation of the ERK1/2 (Fig. 5a) and, secondly, examined whether this kinase may be involved in mediating the differential effects of AICAR treatment on FSH-induced progesterone production in F3/4 and F1 granulosa cells (Fig. 5b and c). As shown in Fig. 5a, AICAR treatment for 24 h caused a dose-dependent increase in the phosphorylations of ERK1/2 in F1 granulosa cells. Similar results were obtained in F3/4 granulosa cells (data not shown). Consistent with previous reports using chicken cells (Soler et al. 1999, Johnson et al. 2001), phosphorylated ERK1/2 appeared as a single band (approximately 42 kDa), likely corresponding to ERK2.
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, left panel). In the presence of FSH, AICAR treatment decreased ERK1/2 phosphorylation in F3/4 granulosa cells, whereas it increased ERK1/2 phosphorylation in F1 granulosa cells. The differential effects of AICAR on ERK1/2 phosphorylation in F3/4 and F1 granulosa cells were abolished by expression of the 1-DN Ad (Fig. 5b and c, right panel), whereas a control GFP virus had no effect (data not shown). Furthermore, the 1-DN treatment alone or in combination with FSH did not have any effects on ERK1/2 phosphorylation (data not shown). The inverse pattern of progesterone secretion and ERK1/2 phosphorylation in our experiments suggests that the former could be regulated by the latter. This was confirmed by showing that the MEK1/2-specific inhibitor U0126, which totally inhibits ERK1/2 phosphorylation (Fig. 6a and b) without altering cell viability increased FSH-induced progesterone secretion by about 3- and 1·3-fold in F3/4 and F1 granulosa cells, respectively (Fig. 6a and b).
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Discussion |
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Thr172 and ACC Ser79 phosphorylation decreases in granulosa cells, but not in theca cells, during follicular development (F4–F1). AMPK activation decreases FSH-induced progesterone secretion through the ERK1/2 MAPK in primary F1 hen granulosa cells, whereas opposite data are observed in granulosa cells from mixed F3 and F4 preovulatory follicles. This is the first report, to our knowledge, showing that AMPK is expressed in the avian ovary and involved in the regulation of ovarian function in this species.
Different ampk subunits (1, 2, ß1, ß2, 1, and 2) mRNA were detected in both granulosa and theca cells of hen follicles. However, the ampk 3 subunit mRNA was not found in hen ovary, whereas it was strongly expressed in hen pectoralis muscle. AMPK1/2 and AMPKß1 and ß2 protein levels were similar in both granulosa and theca cells from follicles at different development stages (F4–F1). The fact that AMPK3 is specifically expressed in muscle is in agreement with some results recently observed in chicken (Proszkowiec-Weglarz et al. 2006) and also in other species, such as human (Cheung et al. 2000) and pig (Milan et al. 2000). In our study, we demonstrated that the activation of AMPK changes during follicle development in granulosa cells but not in theca cells. Indeed, the levels of AMPK Thr172 phosphorylation and those of ACC Ser79 phosphorylation decreased in granulosa cells during maturation of yellow preovulatory follicles (F4–F1). AMPK phosphorylates ACC, a rate-limiting enzyme controlling the conversion of acetyl-CoA to malonyl-CoA; this phosphorylation inhibits the activity of ACC and consequently increases the fatty acid oxidation (Merrill et al. 1997). Thus, we can speculate that the higher phosphorylation of AMPK Thr172 and ACC Ser79 in F4 and F3 than in F1 granulosa cells can lead to higher fatty acid oxidation and consequently higher energy production in these cells. This energy could contribute to rapid development of chicken ovarian follicles. Indeed, hen follicles contain large amount of lipid as yolk. The lower level of both AMPK and ACC phosphorylation and consequently the higher ACC activity in the help F1 follicle could explain the important lipid storage in the highly mature follicle. However, the avian ovary does not synthesize a lot of lipids, all the lipid components of the yolk are derived from plasma precursors, mainly very low density lipoprotein and vitellogenin produced in the liver (Chan 1983). However, granulosa cells express molecules like apolipoprotein A-I (Apo-AI; Hermann et al. 1998) and sterol carrier protein 2 (SCP2; Pfeifer et al. 1993) suggesting a role of these cells in the lipid transport. Furthermore, a flow cytometric analysis of steroidogenic organelles in differentiating hen granulosa cells from F6 to F1 suggests a recruitment of lipids into droplets during the differentiation of granulosa cells (Dive et al. 1992). Thus, AMPK activation could modulate the local lipid transport from granulosa cells either to theca cells or to the growing oocytes. In mammals, AMPK is regulated by different stimuli (cellular stresses, anti-diabetic drugs, and exercise) and hormones including leptin (Kahn et al. 2005). Leptin plays a pivotal role in regulation of energy expenditure (Kahn et al. 2005). In mammals, AMPK activation appears to control leptin’s effects on fatty acid oxidation in muscle (Minokoshi et al. 2002). Thus, some leptin actions on chicken ovarian cells could be mediated through AMPK activation.
Interestingly, we did not detect any variation of AMPK Thr172 and ACC Ser79 phosphorylation in theca cells during follicle development. This differential regulation of AMPK activation between granulosa and theca cells might be partly due to the specificity of their function. Indeed, unlike mammals, progesterone in chickens is synthesized and secreted mainly by granulosa cells, whereas theca cells generate estradiol (Huang et al. 1979). Progesterone produced by granulosa cells from mature follicles provides the positive feedback necessary to stimulate a preovulatory surge of LH (Robinson & Etches 1986). In rat granulosa cells, we have recently shown that AMPK decreases progesterone secretion without affecting estradiol secretion (Tosca et al. 2005). Thus, in hen, a variation of AMPK activation in granulosa cells during the follicular hierarchy might also affect ovarian steroidogenesis. That is why we studied the effects of a potent AMPK activator, AICAR, on progesterone secretion and cell growth in both F3/4 and F1 granulosa cells. We pooled granulosa cells from F3 to F4 to isolate enough cells for a culture. As expected, we have observed that in the basal state (no FSH treatment), F3/4 granulosa cells produced less progesterone than F1 granulosa cells. However, the FSH-induced progesterone secretion was higher in F3/4 granulosa cells. These latter data are in good agreement with the literature, since this decline of FSH-stimulated steroidogenesis during follicle maturation is associated with a decrease in the number of FSH receptors (Ritzhaupt & Bahr 1987) and also the adenylate cyclase activity (Asem & Hertelendy 1985). The basal release of progesterone in F1 cells is, however, higher. In our study, we have demonstrated that AICAR treatment alone increases progesterone production and this was associated with an increase in the levels of StAR and P450scc in both F3/4 and F1 granulosa cells (Fig. 7). StAR is an indispensable component of steroidogenesis within preovulatory follicles (Stocco 2001). This protein facilitates the transfer of cholesterol to the inner mitochondrial membrane, where it undergoes conversion to pregnenolone by the P450scc. As in mammals, the conversion of cholesterol to pregnenolone is the rate-limiting enzymatic step within granulosa cells of the hen ovary (Tilly et al. 1991). In cultured immature granulosa cells from the diethylstilbestrol-primed rat, AICAR treatment reduces progesterone secretion, probably due to a reduction in the level of the HSD3ß protein (Tosca et al. 2005). These different AICAR effects observed between rat and hen granulosa cells on progesterone secretion might be explained by the differentiation state of the cells (rat immature vs hen granulosa cells from preovulatory follicle) or by some species specificities.
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). Moreover, these results were confirmed using an AMPK dominant-negative Ad, suggesting that these different effects of AICAR between F3/4 and F1 granulosa cells on progesterone secretion were mediated by AMPK activation. Using a specific MEK1/2 inhibitor, we have also demonstrated that inhibition of the ERK1/2 MAPK leads to an increase in the FSH-induced progesterone secretion in both F3/4 and F1 granulosa cells. This ability of MAP kinase signaling via ERK1/2 to inhibit gonadotropin-stimulated steroidogenesis has been reported in human granulosa-derived cells (Seger et al. 2001, Tai et al. 2001) and also in cultured prehierarchical follicle hen granulosa cells (Johnson & Bridgham 2001). However, we have recently shown that in rat primary granulosa cells, the U0126 MEK1/2 inhibitor strongly reduced progesterone production (Tosca et al. 2005). Thus, the ERK1/2 MAPK appears to be a positive or negative regulator of progesterone secretion in granulosa cells according to the species and/or the differentiation state of the granulosa cells. In the present study, we showed that in F1 granulosa cells, AICAR treatment reduces progesterone secretion and StAR protein levels, but increases ERK1/2 phosphorylation in response to FSH. Furthermore, we have observed opposite results in F3/4 granulosa cells. This opposite regulation of AICAR-mediated AMPK activation between follicles in response to FSH is striking. However, it is well known that granulosa cells have different protein composition during follicular maturation. For example, the number of FSH receptors is decreased in F1 granulosa cells as compared with F3/4 granulosa cells (Ritzhaupt & Bahr et al. 1987). Thus, we can speculate that AICAR-induced AMPK activation leads to activation or inhibition of molecules that inhibit or activate the ERK1/2 MAPK and consequently modulates progesterone secretion in response to FSH. Interestingly, we observed that 1-DN treatment alone or in combination with FSH did not have significant effects on progesterone secretion, StAR protein levels and ERK1/2 phosphorylation levels in vitro in both F3/4 and F1 granulosa cells. Thus, it appears that in vitro in the basal state, AMPK phosphorylation does not strongly affect steroidogenesis. However, these data do not exclude a role of AMPK in vivo in granulosa cells. In conclusion, AMPK is expressed and active in granulosa and theca cells from preovulatory hen follicles. Furthermore, we have shown that AMPK activation in vivo is reduced in F1 as compared with F3/4 granulosa cells, suggesting a role of this kinase in hen ovarian steroidogenesis. In the basal state, we have demonstrated that AICAR treatment increases in vitro progesterone secretion in both F3/4 and F1 primary granulosa cells. Furthermore, in response to FSH, AICAR-mediated AMPK activation increases progesterone production and StAR protein levels, whereas it decreases ERK1/2 phosphorylation in F3/4 granulosa cells. Opposite results are observed in F1 granulosa cells. Since FSH is a key hormone in follicular maturation, we can speculate that the activation of AMPK in response to different stresses, such as a variation in the nutritional status, might lead to a modification of steroidogenesis and consequently a dysfunction of the follicular hierarchy in the hen.
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Acknowledgements |
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References |
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Bahr JM & Johnson AL 1984 Regulation of the follicular hierarchy and ovulation. Journal of Experimental Zoology 232 495–500.[CrossRef][Web of Science][Medline]
Bolster DR, Crozier SJ, Kimball SR & Jefferson LS 2002 AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through downregulated mammalian target of rapamycin (mTOR). Journal of Biological Chemistry 277 23977–23980.
Carling D 2004 The AMP-activated protein kinase cascade–a unifying system for energy control. Trends in Biochemical Sciences 29 18–24.[CrossRef][Web of Science][Medline]
Carling D, Zammit VA & Hardie DG 1987 A common bicyclic protein kinase cascade inactivates the regulatory enzymes of fatty acid and cholesterol biosynthesis. FEBS Letters 223 217–222.[CrossRef][Web of Science][Medline]
Chan L 1983 Hormonal control of apolipoprotein synthesis. Annual Review of Physiology 45 615–623.[CrossRef][Web of Science][Medline]
Chen HC, Bandyopadhyay G, Sajan MP, Kanoh Y, Standaert M, Farese RV Jr. & Farese RV 2002 Activation of the ERK pathway and atypical protein kinase C isoforms in exercise- and aminoimidazole-4-carboxamide-1-beta-D-riboside (AICAR)-stimulated glucose transport. Journal of Biological Chemistry 277 23554–23562.
Cheung PC, Salt IP, Davies SP, Hardie DG & Carling D 2000 Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochemical Journal 346 659–669.[CrossRef][Web of Science][Medline]
Corton JM, Gillespie JG & Hardie DG 1994 Role of the AMP-activated protein kinase in the cellular stress response. Current Biology 4 315–324.[CrossRef][Web of Science][Medline]
Corton JM, Gillespie JG, Hawley SA & Hardie DG 1995 5-Aminoimidazole-4 carboxamide ribonucleoside. A specific method for activating AMP-activated protein kinase in intact cells? European Journal of Biochemistry 229 558–565.[Web of Science][Medline]
Davies SP, Helps NR, Cohen PT & Hardie DG 1995 5'-AMP inhibits dephosphorylation, as well as promoting phosphorylation, of the AMP-activated protein kinase. Studies using bacterially expressed human protein phosphatase-2C alpha and native bovine protein phosphatase-2AC. FEBS Letters 377 421–425.[CrossRef][Web of Science][Medline]
Dive C, Yoshida TM, Simpson DJ & Marrone BL 1992 Flow cytometric analysis of steroidogenic organelles in differentiating granulosa cells. Biology of Reproduction 47 520–527.[Abstract]
Downs SM, Hudson ER & Hardie DG 2002 A potential role for AMP-activated protein kinase in meiotic induction in mouse oocytes. Developmental Biology 245 200–212.[CrossRef][Web of Science][Medline]
Etches RJ & Duke CE 1984 Progesterone, androstenedione and oestradiol content of theca and granulosa tissues of the four largest ovarian follicles during the ovulatory cycle of the hen (Gallus domesticus). Journal of Endocrinology 103 71–76.
Etches RJ & Petitte JN 1990 Reptilian and avian follicular hierarchies: models for the study of ovarian development. Journal of Experimental Zoology Supplement 4 112–122.
Hammond RW, Todd H & Hertedenly F 1978 Effects of bovine LH on steroidogenesis in avian granulosa cells IRCS (Int. Res. Comm. Syst.). Medical Science Library Compendium 446–452.
Hardie DG 2003 Minireview: the AMP-activated protein kinase cascade: the key sensor of cellular energy status. Endocrinology 144 5179–5183.
Hawley SA, Davison M, Woods A, Davies SP, Beri RK, Carling D & Hardie DG 1996 Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. Journal of Biological Chemistry 271 27879–27887.
Hermann M, Lindstedt KA, Foisner R, Morwald S, Mahon MG, Wandl R, Schneider WJ & Nimpf J 1998 Apolipoprotein A-I production by chicken granulosa cells. FASEB Journal 12 897–903.
Huang ES, Kao KJ & Nalbandov AV 1979 Synthesis of sex steroids by cellular components of chicken follicles. Biology of Reproduction 20 454–461.[Abstract]
Johnson PA & Brooks C 1996 Developmental profile of plasma inhibin and gonadotropins from hatch to sexual maturity in male and female chickens. General and Comparative Endocrinology 102 56–60.[CrossRef][Web of Science][Medline]
Johnson AL & Bridgham JT 2001 Regulation of steroidogenic acute regulatory protein and luteinizing hormone receptor messenger ribonucleic acid in hen granulosa cells. Endocrinology 142 3116–3124.
Johnson AL, Bridgham JT & Swenson JA 2001 Activation of the Akt/protein kinase B signaling pathway is associated with granulosa cell survival. Biology of Reproduction 64 1566–1574.
Johnson AL, Solovieva EV & Bridgham JT 2002 Relationship between steroidogenic acute regulatory protein expression and progesterone production in hen granulosa cells during follicle development. Biology of Reproduction 67 1313–1320.
Kahn BB, Alquier T, Carling D & Hardie DG 2005 AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metabolism 1 15–25.[CrossRef][Web of Science][Medline]
Kemp BE, Mitchelhill KI, Stapleton D, Michell BJ, Chen ZP & Witters LA 1999 Dealing with energy demand: the AMP-activated protein kinase. Trends in Biochemical Sciences 24(22) 25.
Kim J, Yoon MY, Choi SL, Kang I, Kim SS, Kim YS, Choi YK & Ha J 2001 Effects of stimulation of AMP-activated protein kinase on insulin-like growth factor 1- and epidermal growth factor-dependent extracellular signal-regulated kinase pathway. Journal of Biological Chemistry 276 19102–19110.
Larosa C & Downs SM 2006 Stress stimulates adenosine monophosphate-activated protein kinase and meiotic resumption in mouse oocytes. Biology of Reproduction 74 585–592.
Marrone BL & Hertelendy F 1983 Steroidogenesis by avian ovarian cells: effects of luteinizing hormone and substrate availability. American Journal of Physiology 244 E487–E493.[Medline]
Merrill GF, Kurth EJ, Hardie DG & Winder WW 1997 AICA riboside increases AMP-activated protein kinase, fatty acid oxidation, and glucose uptake in rat muscle. American Journal of Physiology 273 E1107–E1112.[Web of Science][Medline]
Milan D, Jeon JT, Looft C, Amarger V, Robic A, Thelander M, Rogel-Gaillard C, Paul S, Iannuccelli N, Rask L et al. 2000 A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. Science 288 1248–1251.
Minokoshi Y, Kim YB, Peroni OD, Fryer LG, Muller C, Carling D & Kahn BB 2002 Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase. Nature 415 339–343.[CrossRef][Medline]
Onagbesan OM, Vleugels B, Buys N, Bruggeman V, Safi M & Decuypere E 1999 Insulin-like growth factors in the regulation of avian ovarian functions. Domestic Animal Endocrinology 17 299–313.[CrossRef][Web of Science][Medline]
Pfeifer SM, Sakuragi N, Ryan A, Johnson AL, Deeley RG, Billheimer JT, Baker ME & Strauss JF 3rd 1993 Chicken sterol carrier protein 2/sterol carrier protein x: cDNA cloning reveals evolutionary conservation of structure and regulated expression. Archives of Biochemistry and Biophysics 304 207–293.
Proszkowiec-Weglarz M, Richards MP, Ramachandran R & McMurtry JP 2006 Characterization of the AMP-activated protein kinase pathway in chickens. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 143 92–106.[CrossRef]
Ritzhaupt LK & Bahr JM 1987 A decrease in FSH receptors of granulosa cells during follicular maturation in the domestic hen. Journal of Endocrinology 115 303–310.
Robinson FE & Etches RJ 1986 Ovarian steroidogenesis during follicular maturation in the domestic fowl (Gallus domesticus). Biology of Reproduction 35 1096–1105.[Abstract]
Seger R, Hanoch T, Rosenberg R, Dantes A, Merz WE, Strauss JF 3rd & Amsterdam A 2001 The ERK signaling cascade inhibits gonadotropin-stimulated steroidogenesis. Journal of Biological Chemistry 276 13957–13964.
Soler RM, Dolcet X, Encinas M, Egea J, Bayascas JR & Comella JX 1999 Receptors of the glial cell line-derived neurotrophic factor family of neurotrophic factors signal cell survival through the phosphatidylinositol 3-kinase pathway in spinal cord motor neurons. Journal of Neuroscience 19 9160–9169.
Stapleton D, Woollatt E, Mitchelhill KI, Nicholl JK, Fernandez CS, Michell BJ, Witters LA, Power DA, Sutherland GR & Kemp BE 1997 AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location. FEBS Letters 409 452–456.[CrossRef][Web of Science][Medline]
Stocco DM 2001 StAR protein and the regulation of steroid hormone biosynthesis. Annual Review of Physiology 63 193–213.[CrossRef][Web of Science][Medline]
Tai CJ, Kang SK, Tzeng CR & Leung PC 2001 Adenosine triphosphate activates mitogen-activated protein kinase in human granulosa-luteal cells. Endocrinology 142 1554–1560.
Tilly JL, Kowalski KI & Johnson AL 1991 Cytochrome P450 side-chain cleavage (P450scc) in the hen ovary. II. P450scc messenger RNA, immunoreactive protein, and enzyme activity in developing granulosa cells. Biology of Reproduction 45 967–974.[Abstract]
Tosca L, Froment P, Solnais P, Ferre P, Foufelle F & Dupont J 2005 Adenosine 5'-monophosphate-activated protein kinase regulates progesterone secretion in rat granulosa cells. Endocrinology 146 4500–4513.
Unger RH 2004 The hyperleptinemia of obesity-regulator of caloric surpluses. Cell 117 145–146.[CrossRef][Web of Science][Medline]
Woods A, Cheung PC, Smith FC, Davison MD, Scott J, Beri RK & Carling D 1996 Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro. Journal of Biological Chemistry 271 10282–10290.
Woods A, Azzout-Marniche D, Foretz M, Stein SC, Lemarchand P, Ferre P, Foufelle F & Carling D 2000 Characterization of the role of AMP-activated protein kinase in the regulation of glucose-activated gene expression using constitutively active and dominant negative forms of the kinase. Molecular and Cellular Biology 20 6704–6711.
Woods DC, Haugen MJ & Johnson AL 2005 Opposing actions of TGFbeta and MAP kinase signaling in undifferentiated hen granulosa cells. Biochemical and Biophysical Research Communications 336 450–457.[CrossRef][Web of Science][Medline]
Received in final form 5 April 2006
Accepted 6 April 2006
Made available online as an Accepted Preprint 26 April 2006
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