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This Article Accepted manuscript (PDF) All Versions of this Article: JOE-09-0066v1 202/2/223    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hazell, G. Articles by Lolait, S. Search for Related Content PubMed PubMed Citation Articles by Hazell, G. Articles by Lolait, S.

G Hazell, CSSB HW-LINE DHB, University of Bristol, Bristol, United Kingdom
S Yao, CSSB HW-LINE DHB, University of Bristol, Bristol, United Kingdom
J Roper, CSSB HW-LINE DHB, University of Bristol, Bristol, United Kingdom
E Prossnitz, Cell Biology and Physiology, University of New Mexico, Albuquerque, United States
A O'Carroll, CSSB HW-LINE DHB, University of Bristol, Bristol, United Kingdom
S Lolait, Clinical Sciences@South Bristol, University of Bristol, Bristol, BS1 3NY, United Kingdom

Correspondence: Steve Lolait, Email: S.J.Lolait{at}bristol.ac.uk

Recently the G protein-coupled receptor GPR30 has been identified as a novel oestrogen receptor. The distribution of the receptor has thus far been mapped only in the rat central nervous system. This study was undertaken to map the distribution of GPR30 in the mouse brain, and rodent peripheral tissues. Immunohistochemistry using an antibody against GPR30 revealed high levels of GPR30 immunoreactivity (ir) in the forebrain (e.g., cortex, hypothalamus and hippocampus), specific nuclei of the midbrain (e.g., the pontine nuclei and locus coeruleus) and the trigeminal nuclei and cerebellum Purkinje layer of the hindbrain in the adult mouse brain. In the rat and mouse periphery GPR30-ir was detected in the anterior, intermediate and neural lobe of the pituitary, adrenal medulla, renal pelvis and ovary. In situ hybridisation histochemistry using GPR30 riboprobes, revealed intense hybridisation signal for GPR30 in the paraventricular nucleus and supraoptic nucleus of the hypothalamus, anterior and intermediate lobe of the pituitary, adrenal medulla, renal pelvis and ovary of both rat and mouse. Double immunofluorescence revealed GPR30 was present in both oxytocin and vasopressin neurones of the paraventricular nucleus and supraoptic nucleus of the rat and mouse brain. The distribution of GPR30 is distinct from the other traditional oestrogen receptors and offers an additional way in which oestrogen may mediate its effects in numerous brain regions and endocrine systems in the rodent.