HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Accepted manuscript (PDF) All Versions of this Article: JOE-07-0428v1 198/1/157    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Stoppa, G. Articles by Torsoni, M. PubMed PubMed Citation Articles by Stoppa, G. Articles by Torsoni, M.

G Stoppa, Bioquímica, UNICAMP, Campinas, Brazil
M Cesquini, Bioquímica, UNICAMP, Campinas, Brazil
E Roman, Bioquímica, UNICAMP, Campinas, Brazil
P Prada, Clinica Médica, UNICAMP, Campinas, Brazil
A Torsoni, Area da Saude, Universidade Braz Cubas, Mogi das Cruzes, Brazil
T Romanatto, Clinica Médica, UNICAMP, Campinas, Brazil
M Saad, Clinica Médica, UNICAMP, Campinas, Brazil
L Velloso, Campinas, Brazil
M Torsoni, Área da Saude, Universidade Braz Cubas, Mogi das Cruzes, 08773-380, Brazil

Correspondence: Marcio Torsoni, Email: torsoni{at}yahoo.com

Abstract

We hypothesized that citrate might modulate the AMPK/ACC pathway and participate in neuronal feeding control and glucose homeostasis. To address this issue, we injected citrate into the lateral ventricle of rats. Intracerebroventricular (ICV) injection of citrate diminished phosphorylation of hypothalamic AMPK/ACC, increased anorexigenic neuropeptide (POMC and CRH) expression, and reduced food intake. With euglycemic hyperinsulinemic clamp, the rate of glucose infusion was higher in the citrate group than in the control group (28.6 ± 0.8 vs 19.3 ± 0.2 mU/kg body weight/min, respectively), as was glucose uptake in skeletal muscle and the epididymal fat pad. Concordantly, IR, IRS1, IRS2, and AKT phosphorylation in adipose tissue and skeletal muscle were improved by citrate ICV treatment. Moreover, the treatment with citrate for seven days promoted body weight loss and decreased the adipose tissue. Our results suggest that citrate as well as glucose may serve to hypothalamus as cell signals of energy and nutrient availability.