HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lahtonen, R. Articles by Vihko, R. Search for Related Content PubMed Articles by Lahtonen, R. Articles by Vihko, R.

Prostate tissues removed from patients with benign prostatic hypertrophy were separated into epithelial and stromal components and nuclei purified from these. The concentrations of testosterone, 5-dihydrotestosterone, 5-androstane-3,17β-diol, 4-androstene-3,17-dione, 5-androstanedione and androsterone in pooled preparations of the purified nuclei were determined by radioimmunoassays after the purification of solvent steroid extracts by Lipidex-5000 column chromatography. The most abundant androgen measured was 5-dihydrotestosterone and it was significantly (P < 0·05) more concentrated in the stromal nuclei than in the epithelial nuclei. The mean concentration of androsterone was also significantly (P <0·05) greater in the stromal nuclei whereas that of testosterone was equal in the two nuclear types. The concentrations of 5-androstane-3,17β-diol, 4-androstene-3,17-dione and 5-androstanedione were below the sensitivity limits of the assays in the majority of cases, but the results indicated that when detectable the first two were more concentrated in the stromal than the epithelial nuclei. The results emphasize the importance of the prostatic stroma in androgen metabolism, and the relative concentrations of 5-dihydrotestosterone and testosterone indicated identical 5-reductase activities in the nuclei in comparison with respective whole cell epithelial and stromal preparations. 5-Dihydrotestosterone concentrations were clearly higher than the nuclear androgen receptor levels previously reported from this laboratory.