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Journal of Endocrinology (1983) 98, 381-384    DOI: 10.1677/joe.0.0980381
© 1983 Society for Endocrinology
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Glycerol prevents loss of immunoreactive follicle-stimulating hormone and luteinizing hormone from frozen urine

 J. H. Livesey, H. K. Roud, M. G. Metcalf and R. A. Donald

First morning urine samples were collected from both menstruant and post-menopausal women and stored at –25 °C. Immunoreactive FSH disappeared from these samples (t1/2 = 30 days), ultimately stabilizing at about 20% of the initial value. The loss was more rapid at –20 °C and less rapid at –55 °C and +4°C. Immunoreactive LH was also lost from frozen urine, but more slowly than FSH. The addition of glycerol to urine (0·52 mol/l) stored at –25 °C prevented loss of immunoreactive FSH and LH for at least 105 days.




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