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Hybridization of RNA to the uteroglobin cDNA probe: RNA-excess hybridization was performed in a final volume of 25 µl in 0·6 M-NaCl, 10 mM-Hepes, pH 7·0 and 2 mM-EDTA, as described by Harris, Schwartz, Tsai, Roy & O'Malley (1976). [3H]-Labelled single-stranded uteroglobin cDNA (0·2 ng) was boiled together with RNA (0·6–2000 µg/ml) for 10 min. The mixture was then incubated at 68 °C for intervals ranging from 3 min to 72 h. After hybridization, the samples were treated with S1 nuclease (8000 units) and S1 nuclease-resistant hybrids were determined by precipitation with 10% trichloroacetic acid, as described by Harris et al. (1976). The RNA concentration x time (R0t) values were converted to equivalent values
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