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After administration of an inhibitor of 3β-hydroxysteroid dehydrogenase activity to late-pregnant sheep, a sharp and significant fall in progesterone concentrations in peripheral plasma was noted, and lowered levels were sustained for up to 24 h. However, no significant change in the secretion of ovine placental lactogen (oPL) was noted. These results indicate that progesterone secretion does not regulate secretion of oPL during pregnancy.