HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Daniel, S. G. Articles by Ramsden, D. B. Search for Related Content PubMed Articles by Daniel, S. G. Articles by Ramsden, D. B.

The release of immunoreactive β-endorphin from rat anterior pituitary cells in culture was studied by radioimmunoassay and gel-filtration chromatography. Two forms of immunoreactivity were detected corresponding to β-lipotrophin (β-LPH) and β-endorphin. Cells were found to release β-endorphin-like immunoreactivity (β-endorphin LIR) into the culture medium for up to 10 days with a trough in release occurring between days 2–4. The ratio of β-LPH to β-endorphin released remained constant during the course of culture. After 4 days in culture β-endorphin LIR release was constant and responsive to modulation. Incubation with lysine-vasopressin (0·1 units/ml) produced a two- to threefold increase in release and dexamethasone (10^–6 mol/l) suppressed release to 33% of controls. Neither stimulation nor suppression resulted in a change in the ratio of β-LPH to β-endorphin released. Dexamethasone suppression was not overcome by removal of dexamethasone or addition of vasopressin.