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Plasma somatomedin activity enhanced the incorporation of [³H]thymidine into chondrocytes isolated from human foetal cartilage during weeks 13–21 of gestation. Human growth hormone (0·1–20 µu./ml), human placental lactogen (0·1–5 µg/ml) and insulin (0·25– 10 µu./ml) had no direct effects on the synthesis of DNA in these chondrocytes, although insulin at concentrations of 2·5–100 mu./ml increased [³H]thymidine incorporation by up to 400%.