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Nuclei have been isolated from canine prostatic tissue previously incubated with tritiated testosterone or tritiated 5-androstane-3,17-diol. The protein-bound radioactivity has, in both cases, been shown to be associated with 5-androstane-3,17-diol. The formation and translocation of this steroid to the nucleus supports previous evidence that it has an active part to play in prostatic function in the dog. The formation from testosterone of another tritiated steroid, possibly 5-dihydrotestosterone, was also demonstrated although this product did not enter the nucleus and was apparently bound to the nuclear membrane.