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The effects of sodium oestrone sulphate on the uterus of the mouse in vitro have been investigated.

Motor effects are produced on the uterus of (1) oestrous animals and (2) ovariectomized animals treated with oestradiol and certain other steroids. Inhibitory effects are produced on the uterus of (1) metoestrous animals, (2) pregnant animals and (3) ovariectomized animals treated with progesterone, testosterone and certain other steroids. The antagonism between oestradiol on the one hand, and progesterone and testosterone on the other, has been studied.

Steroids which produce a condition of the uterus in which it is inhibited by sodium oestrone sulphate also inhibit the vaginal response to oestrogen in ovariectomized mice.