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Typical urinary phenols and oestrogens have been converted into azo dyes by coupling with diazotized I.C.I. M5091.

The dyes have been characterized from the following data:

(a) partition properties;

(b) solubility properties in a phosphate buffer;

(c) adsorption properties;

(d) absorption spectra data 310–600 mµ.

Decalso, after treatment with barium acetate solution, has been introduced as a new adsorbent in chromatography.

The removal of indigoid pigments from urinary oestriol fractions by a new procedure is described.

The addition of thiourea and benzene to urines during acid hydrolysis affords some protection to oestradiol-3:17.

This article has been cited by other articles:

				J. B. Garst and H. B. Friedgood On the Nature of the So-Called Background Material in Estrogen Fractions of Extracts Prepared from Hydrolyzed Urine Science, July 18, 1952; 116(3003): 65 - 66. [PDF]