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Departments of Pharmacology and Medicine, University of Bristol Medical School, Bristol, BS8 1TD

(Received 3 February 1975)

In a previous study (Sharpe, Shahmanesh, Ellwood, Hartog & Brown, 1975) pituitary glands from male rats of various ages were incubated with or without the addition of synthetic luteinizing hormone releasing hormone (LH-RH). The luteinizing hormone (LH) released was measured by radioimmunoassay (RIA), radioligand receptor-binding assay and bioassay. The RIA underestimated the LH released compared with the other two methods, the discrepancy being greatest when pituitaries from animals aged 40 or 60 days were stimulated with LH-RH. Since a similar comparison for follicle-stimulating hormone (FSH) release has not been reported, this paper presents results obtained when the FSH released under similar experimental conditions was measured by both RIA and bioassay.

Anterior pituitaries were incubated as previously described with and without added LH-RH in doses of 2 or 20 ng/ml (Sharpe et al. 1975).