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Department of Anatomy, King's College, The Strand, London, WC2R 2LS

(Received 13 January 1975)

It has been found that the incubated posterior pituitary of the mouse is capable of incorporating [³H]phenylalanine into corticotrophin (ACTH), and also that active ACTH synthesis occurred under conditions when no synthesis of melanocyte-stimulating hormone (MSH) was taking place, suggesting that synthesis of each hormone is regulated separately.

Agouti mice (CBA/Ca strain) of both sexes were used. Posterior pituitaries were dissected free from anterior pituitary tissue and, in groups of 8–10, incubated at 37 °C in 1 ml bicarbonate-buffered Krebs–Ringer solution gassed with O₂ : CO₂ (95:5, v/v) and containing 1 mg bovine serum albumin and 20 µCi (2 nmol) L-[4-³H] phenylalanine. After 30 min, the radioactive medium was removed and replaced by 1 ml Krebs–Ringer solution containing albumin and 1 µmol L-phenylalanine. After 5 min, the glands were removed and homogenized together in 0·2 ml ice-cold