HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by JORDAN, V. C. Articles by KOERNER, S. Search for Related Content PubMed PubMed Citation Articles by JORDAN, V. C. Articles by KOERNER, S.

Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545, U.S.A.

(Received 28 August 1974)

Harper & Walpole (1967 reported the potent antioestrogenic activity of ICI 46,474, the trans-isomer of 1-(p-β-dimethylaminoethoxyphenyl)-1,2-diphenylbut-1-ene, in the rat; however, in the mouse the compound exhibited only oestrogenic properties. However, Emmens (1971) showed that large doses of ICI 46,474 or the related compound H774 (1-(p-β-diethylaminoethoxyphenyl)-1,2-di(p-methoxyphenyl)-but-1-ene citrate) caused vaginal refractoriness to oestradiol for several weeks after s.c. administration to ovariectomized mice. The present study was undertaken to determine the ability of ICI 46,474 and H774 to inhibit binding of [³H]oestradiol to the 8 S oestrogen receptor derived from ovariectomized mouse uterus and vagina.

Mature, Charges River CD strain mice were ovariectomized under ether anaesthesia, primed with 1 µg oestradiol-17β in 0·05 ml peanut oil and used for experimentation 2 weeks later. Animals were killed by cervical dislocation and the uteri and vaginae were dissected out, weighed and immediately

This article has been cited by other articles:

				S. F. Eisen and H. A. Brown Selective Estrogen Receptor (ER) Modulators Differentially Regulate Phospholipase D Catalytic Activity in ER-Negative Breast Cancer Cells Mol. Pharmacol., October 1, 2002; 62(4): 911 - 920. [Abstract] [Full Text] [PDF]