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A number of methods have been proposed for the biological assay of human chorionic gonadotrophin (C.G.). The test animals have usually been immature or hypophysectomized rats or mice in which circulating gonadotrophins were lacking.

The index of response has varied greatly [Thayer, 1946]. Tests have been based on observations of corpora lutea [Aschheim & Zondek, 1927; Emmens, 1939; Kennedy, 1933; Siegler, 1939; Smith & Smith, 1934, 1935, 1939, 1948; White, 1937; White & Hunt, 1943]; ovarian weight [Evans, Kohls & Wonder, 1937; Evans, Meyer & Simpson, 1929, 1931, 1933; Wallen-Lawrence & Van Dyke, 1931]; uterine weight [Delfs, 1941, Emmens, 1939; Dorfman, Rubin & Miller, 1946, 1947; Jones, Delfs & Stran, 1944; Sealey & Sondern, 1940]; vaginal smears [Browne & Venning, 1936a, b, 1938; Venning, 1948; Venning & Browne, 1936; Wallen-Lawrence & Van Dyke, 1931]; vaginal smears in vitamin B-deficient rats [Heard, 1941; Heard & Winton, 1939]; seminal vesicles [Bischoff,