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After adrenalectomy the non-diffusible molecule content of the skeletal muscles, liver and testis changes. The idiomolar value () decreases in each case, while the electrostatic equivalent () increases in skeletal muscle only [Conway & Hingerty, 1946; Cole, 1949]. The effects of deoxycorticosterone acetate (DOCA) on the electrolyte, water and non-diffusible molecule content of the corresponding tissues from adrenalectomized animals have now been investigated.

MATERIAL AND METHODS: Fourteen male albino rats were used; all the animals were adrenalectomized by the lumbar approach, using ether anaesthesia. The rats were fed on a stock diet consisting of oat meal and oat flour, reinforced with 'Bemax', cod-liver oil and crystalline ascorbic acid, and were kept in a room at 18–21° C. after the operation. The diet, which appeared deficient without vitamin concentrates, was satisfactory after their addition. All the animals had free access to food and water.

The animals were killed 5 days after