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Considerable interest attaches to the attempted histochemical demonstration of ketosteroids in adrenal tissue. Phenylhydrazine was the first reagent used for this purpose [Bennett, 1940]. Dempsey & Wislocki [1946] later suggested that 2:4-dinitro-phenylhydrazine (D.P.H.) was more satisfactory, and it has been used extensively by many investigators. There is now evidence, however, that Bennett's reagent is not specific for ketones, and that the phenylhydrazones are formed by a condensation of tissue aldehydes with phenylhydrazine [Gomori, 1942].

Nevertheless, this observation does not exclude the possibility that part, at any rate, of the reaction is due to ketones. In the study reported in this paper an attempt was made by means of the amine-aldehyde condensation of Oster & Mulinos [1944] to render the D.P.H. reaction more specific for ketones by eliminating aldehydes at the start. A negative D.P.H. reaction was obtained after this treatment. This indicates that Gomori's [1942] criticisms of the views advanced