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Changes in the peripheral plasma level of oestradiol during the bovine oestrous cycle were determined by a competitive protein-binding assay, following chromatographic purification of the steroid. Oestradiol concentration increased during the 3 days preceding oestrus and attained its peak value [17 ± 1·9 (S.E.M.) ng/100 ml] 4 h before oestrous behaviour could be detected. The level declined steeply during the day of oestrus to reach a nadir (0·8 ± 0·11) before the time of ovulation. A minor rise was observed on day 4 and a more sustained increase on days 10–13, with a peak on day 11 (8·1 ± 3·6). Comparison with published data on luteinizing hormone (LH) levels suggests that the pre-oestrous surge of oestradiol precedes the pre-ovulatory surge of LH in the cow.

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				C. A. Lents, F. J. White, N. H. Ciccioli, R. P. Wettemann, L. J. Spicer, and D. L. Lalman Effects of body condition score at parturition and postpartum protein supplementation on estrous behavior and size of the dominant follicle in beef cows J Anim Sci, October 1, 2008; 86(10): 2549 - 2556. [Abstract] [Full Text] [PDF]