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Journal of Endocrinology (1972) 55, 203-204    DOI: 10.1677/joe.0.0550203
© 1972 Society for Endocrinology

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THE TIME-COURSE OF THE MITOTIC RESPONSE TO OESTROGEN IN THE EPITHELIA AND STROMA OF THE MOUSE UTERUS

R. M. DAS

The number of cells that synthesize DNA in the uterine luminal epithelium of ovariectomized mice and in the uterine connective tissue stroma of progesterone-treated mice reaches a maximum approximately 15 h after oestrogen is given (Martin, Finn & Trinder, 1973a, b). Martin & Finn (1968) maintained that the time-course of the mitotic response was similar in both tissues, but DNA synthesis has been shown to be of 3 h shorter duration in the oestrogen-stimulated epithelium than in the stroma (Das, 1972), and on this basis the time-courses of the mitotic responses might be expected to differ.

Albino mice were ovariectomized under tribromoethanol (Avertin) anaesthesia. Five days later they were primed with 100 ng oestradiol s.c. for 3 days. Starting 2 days after priming, groups of five mice received daily injections of 1 mg progesterone s.c. for 4 days, and 50 ng oestradiol were given with the last injection. Other




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