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A technique is described by which the unidirectional influx of sodium into toad bladder mucosal cells can be measured over short periods.

The uptake of sodium, which is linear over a 60 s contact period, changes in a non-linear way when the external sodium concentration is varied from 5 to 115mM.

Vasotocin applied to the serosal surface increases significantly the influx of sodium; amiloride applied to the mucosal surface inhibits influx. This is true whether high or low concentrations of sodium are used to bathe the mucosal surface.

The kinetics for the unidirectional influx of sodium provide direct evidence that the passage of sodium into this tissue is by some means other than simple diffusion.