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The concentrations of oestrone and oestradiol in jugular vein plasma during the bovine oestrous cycle were measured using a fluorescence assay. The highest levels of both oestrogens were found on the 19th and 20th days of a standardized 21-day cycle, with the peak of oestrone occurring slightly before that of oestradiol. Oestrone values ranged from 23·1 to 226·9 ng/100ml and oestradiol varied between 17·6 and 117·5 ng/100 ml. Relatively high values for oestrone concentrations were found on days 6, 7 and 8. These values are comparable to those observed in the ovarian vein blood of the ewe, using both the present method and radioimmunoassay.

The relationship between the oestrogen concentrations reported here and the levels of other hormones during the bovine oestrous cycle is discussed.