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Castrated male mice were injected with 250 µg testosterone propionate on 3 consecutive days. With the third injection, 1 µCi tritiated thymidine/g body weight was given intraperitoneally. A pulse-chase analysis in the seminal vesicle and coagulating gland was performed during the next 50 h and labelling and mitotic indices were obtained.

One hour after the injection of tritiated thymidine the labelling index was 19% in the seminal vesicle and 13% in the coagulating gland. The mitotic index was 1·7% in the seminal vesicle and 1·4% in the coagulating gland.

The observed cell cycle (T_c) duration in the seminal vesicle was 25 h, and the duration of DNA synthesis (t_s) was 9 h. The calculated duration of mitosis (t_m) was 0·6 h, the presynthetic duration (t_G1) was 14·2 h, and the postsynthetic duration (t_G2) 1·2 h. Forty-eight hours after testosterone propionate the growth fraction was 0·62 and a decycling ratio of 0·8 was calculated. In the coagulating gland T_c was 52·0 h, t_s 9·0 h, t_m 1·0 h, t_g1 41·0 h and t_G2 1·0 h.

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