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A method is described for the isolation of a purified luteinizing hormone (LH) from the anterior lobes of porcine pituitary glands. This includes fractional precipitation, gel filtration and isoelectric focusing. The preparation, which can be recovered in a fairly short time, is of high potency (equivalent to 3·4 mg standard NIH-LH-B7/mg and 6800 i.u. LH/mg).