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Journal of Endocrinology (1971) 51, 771-NP       DOI: 10.1677/joe.0.0510771
© 1971 Society for Endocrinology
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BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

 J. BÍLEK, I. MIKULÁS, J. SLABA and I. SKÁLA

A method for the bioassay of oxytocin in the lactating rat mammary gland in vitro is described. The changes in transparency induced by oxytocin in a flat segment of the gland are recorded by means of a CdS photoresistor and a simple Wheatstone bridge. The sensitivity of the assay in routine use is about 0·5–10 µu. oxytocin, the index of precision being about 0·1.






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