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Hypophysectomized—thyroidectomized geckos failed to slough during the experimental period of about 120 days. The resting phase of the sloughing cycle persisted so that the outer epidermal generation remained incomplete. These results indicate that one important endocrine component of the sloughing cycle is the normal functioning of the pituitary—thyroid system.

Prolactin administration at 0·4 i.u./g body wt on alternate days restored sloughing by reducing the length of the resting phase; the outer generation was completed and was shed after the appearance of a new inner unit.