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An attempt was made to isolate dihydrotestosterone (5-androstan-17β-ol-3-one) from samples of testicular tissue taken from a 3-yr-old stallion and a mature bull. The extracts were quantitated by gas—liquid chromatography. In neither case was it possible to detect dihydrotestosterone.

In addition, the androgenic potency of dihydrotestosterone in vivo was compared with that of testosterone. Castrated rats received 14 daily injections subcutaneously of 300 µg of steroid in 0·1 ml arachis oil. After treatment, the rats were killed and the ventral prostates, seminal vesicles and coagulating glands were removed from each rat and weighed. Each gland was subsequently examined histologically. The fructose content of the coagulating glands was measured. The glands taken from rats treated with dihydrotestosterone differed in no way from those of rats treated with testosterone.