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The use of the resting plasma cortisol level, the diurnal plasma cortisol rhythm and plasma cortisol response to a maximal adrenocorticotrophic hormone (ACTH) infusion or insulin-induced hypoglycaemia as indirect tests of hypothalamo—pituitary—adrenocortical function was examined in a group of 27 patients with pituitary disease. While the first three tests provided similar information about hypothalamo—pituitary—adrenocortical function, the plasma cortisol response to insulin-induced hypoglycaemia did not provide further clinically useful information. Difficulties in interpretation of dynamic tests of hypothalamo—pituitary—adrenocortical function are discussed.