HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by SNART, R. S. Articles by AGARWAL, M. K. Search for Related Content PubMed PubMed Citation Articles by SNART, R. S. Articles by AGARWAL, M. K.

The binding characteristics of corticosterone by rat liver were studied by a displaceable binding technique. The binding of corticosterone to protein fractionated by gel filtration and density gradient centrifugation has been carried out as a preliminary determination of the nature of the binding sites. The results were analysed and showed three types of binding sites for corticosterone with the characteristic association constants at 0° of K₁ = 1·2 x 10¹⁰, K₂ = 1 x 10⁸ and K₃ = 1 x 10⁴ 1./mole. Percentage displacement of corticosterone from the nuclear fraction did not differ significantly from that from tissue or the mitochondrial-microsomal fraction. The K₁ and K₂ sites persisted in separated buffer-soluble fractions but were destroyed on mild heating leaving only the K₃ sites.