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Oestriol concentrations in amniotic fluid have been investigated by several workers as a possible means of assessing foeto-placental function (Berman, Kalchman, Chattoraj & Scommegna, 1968; Michie & Livingstone, 1969). Certain basic data are required, however, in working with any biological fluid and although information is available for storage effects on urinary oestriol (Leon, Bulbrook & Greenwood, 1959) more information is needed for amniotic fluid. This paper describes an investigation of the effects of storage at different temperatures, of filtration and of the influence of glucose concentration on amniotic fluid oestriol using a gas-liquid chromatographic (GLC) method.
Amniotic fluid was obtained at surgical induction of labour in patients in the 40th or 41st weeks of normal pregnancy. For the study of storage effects, 450 ml. of amniotic fluid was obtained from one patient and immediately divided into three parts. The first was stored in a stoppered flask at room temperature (22°),
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