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Autoradiography of human skin, after incubation with tritiated aldosterone or pregnenolone, showed both steroids to be concentrated in all types of cells. Binding of [³H]aldosterone in skin was investigated in the presence and absence of a large excess of unlabelled hormone. The capacity of the cells to bind aldosterone in a manner suggesting attachment to receptor sites was estimated to be 5 x 10^-13 moles/g. The rate of RNA synthesis was measured in the various cells of the sweat gland: it was shown to be significantly increased by aldosterone. This stimulation was observed in the inner and outer cells of the sweat duct and probably also in the secretory tubule.