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Recent studies in which [7-³H]dehydroepiandrosterone sulphate (DHA-S) was shown to be converted to oestrogen by minced corpora lutea (Fahmy, Griffiths & Turnbull, 1968a), also showed the formation of 19-hydroxyandrostenedione (19-OH-A) but not 19-hydroxytestosterone (19-OH-T). Little is known concerning the role of testosterone as a direct precursor of oestradiol-17β synthesis in the ovary, and of 19-OH-T as an obligatory intermediate. Even in the placenta the role of 19-OH-T remains equivocal. Perfusion studies of Bolté, Mancuso, Dray, Baulieu & Diczfalusy (1964) suggested that testosterone was directly converted to oestradiol-17β, whereas incubation studies have produced contradictory results (Baulieu, Wallace & Lieberman, 1963; Menini & Engel, 1967). It was therefore decided to investigate further the precursor role of 19-OH-A and 19-OH-T in human luteal tissue.

Radioactive 19-OH-A and 19-OH-T were prepared by incubating either [4-¹⁴C]- or [7-³H]androstenedione with golden hamster adrenal homogenates (Griffiths & Giles, 1965). Incubations