HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by SNEDDON, A. Search for Related Content PubMed Articles by SNEDDON, A.

A comparison has been made between the method of Thomas & Bulbrook (1964, 1966) for estimation of urinary 11-deoxy-17-oxosteroids and a modification of this procedure, utilizing isotopically labelled internal standards to assess accuracy.

To each urine sample was added 45·0 mµc each of [4-¹⁴C]dehydroepiandrosterone and [7-³H]aetiocholanolone. Steroid conjugates were then extracted, hydrolysed and washed with sodium dithionate as described by Thomas & Bulbrook (1964). Separation of aetiocholanolone from dehydroepiandrosterone (DHA) plus androsterone was effected by thin-layer chromatography on silica-gel-G. The plate was developed for 2 hr. in a continuous elution tank in the system pentane:ether (4:1), which removes 'lipid-like' material to the solvent front area of the plate without moving steroids from the origin and gives much more reproducible migration of steroids when the plate is subsequently developed for 2 hr. in a continuous elution tank, using the system benzene:ethyl acetate (60:40). The plate