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Equine granulosa cell cultures were incubated with various labelled steroid hormone precursors, and the products of these incubations were purified and characterized by a combination of paper chromatography, derivative formation and recrystallization. Sodium acetate, cholesterol and pregnenolone were converted mainly to progesterone in addition to significant amounts of 20-hydroxypregn-4-en-3-one and 17-hydroxyprogesterone plus small amounts of dehydroepiandrosterone, androstenedione and oestradiol-17β.

Both androstenedione and testosterone were converted to oestrone and oestradiol-17β. Testosterone was converted to androstenedione but the converse did not apply. These experiments demonstrated that equine granulosa cells in culture contain all the enzymes for converting acetate to progesterone and oestradiol-17β and that the pathways are similar to those of luteal tissue.