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The effects of human growth hormone on carbohydrate and protein metabolism have been studied in monolayer tissue cultures of mouse strain L cells and human dermal fibroblasts. In strain L cell cultures, the hormone consistently caused an increase in cell number, protein synthesis, glucose consumption, and lactic acid and keto acid accumulation in the medium; in human fibroblast cultures it increased only glucose consumption and lactic acid accumulation. It affected none of these parameters in HeLa cells.

Ovine growth hormone had no consistent effect on strain L cells but caused a striking increase in glucose consumption and lactic acid accumulation in the medium of human fibroblasts without changing cell number or protein synthesis.