HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by BROVETTO, J. Articles by COCH, J. A. Search for Related Content PubMed Articles by BROVETTO, J. Articles by COCH, J. A.

In an investigation on the isolation of oxytocin from the blood of lactating women (Coch, Fielitz, Brovetto, Cabot, Coda & Fraga, 1967) the hormone was assayed by measuring its effect on a strip of rat mammary gland. This preparation is suitable for detecting very small amounts of oxytocin, but it is also very sensitive to acetylcholine, adrenaline, noradrehaline, lysine vasopressin, histamine and to a lesser extent to 5-hydroxytryptamine (Smith, 1961; Rydén & Sjoholm, 1962). The separation of oxytocin from these interfering substances by paper chromatography or paper electrophoresis is now reported.

Since hypertensin and bradykinin could also have been present in the blood extract used, we studied the effect of these drugs on a strip of rat mammary gland and found that neither of these peptides produced a measurable effect, even at concentrations as high as 1 µg./ml.

For the separations Whatman 3 mm. filter paper was used, purified by