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The purpose of this work was to apply the thyroid vein catheterization technique to the measurement of some metabolic parameters of the dog's thyroid gland in vivo, and to compare these measurements with corresponding findings obtained in vitro.

The femoral artery and the inferior vein of each thyroid lobe of the dog were catheterized and blood was collected for 2–6 hr. (Dumont & Rocmans, 1964); other small thyroid veins were ligatured. After this period the thyroid was removed, weighed, and partly used for studies in vitro in triplicate incubation flasks. The techniques used for the preparation, incubation, and metabolic evaluation of thyroid slices have been previously described (Dumont, 1964; Dumont & Tondeur-Montenez, 1965). The concentration of glucose in blood and in the incubation medium was measured according to Hoffman (1937) and that of lactate according to Hohorst (1963). Lactate concentration in blood was calculated from the plasma concentration and the