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The variation in the number of eggs shed by mice in response to treatment with gonadotrophic hormones depends on the sexual maturity and genetic constitution of the treated females, on the amount of pregnant mare serum used, and on the number of injections into which the total dose is divided. Dividing the dose into six administrations spread over 24 hr. nearly halved the variability of the response in one strain, and reduced the variance sixfold in another.