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Incubation, with [4-¹⁴C]progesterone, of adrenocortical tissue (posterior cardinal vein preparations) from the eel yielded ¹⁴C-labelled cortisol and cortisone. These two hormones, containing both ¹⁴C and ³H, appeared when similar preparations were incubated simultaneously with [4-¹⁴C]progesterone and [16-³H]pregnenolone. In addition, the following intermediaries were isolated: [¹⁴C, ³H]progesterone; [¹⁴C, ³H]17-hydroxyprogesterone; [³H]17-hydroxypregnenolone and [¹⁴C, ³H]21-deoxycortisol. Analysis of the isotope content of the end products and isolated intermediaries seemed to indicate that the transformation of pregnenolone to corticosteroids proceeded to a large extent through intermediaries other than progesterone. In none of these experiments could the formation of aldosterone be demonstrated. Similarly, incubation of eel adrenocortical tissue with a mixture of [4-¹⁴C]-progesterone and [1,2-³H]corticosterone failed to yield detectable aldosterone.

A further search for aldosterone, using a large amount of eel adrenocortical tissue with [4-¹⁴C]progesterone and [16-³H]pregnenolone as substrates with added angiotensin, also gave negative results. In similar preparations, [1-¹⁴C]sodium acetate was not transformed to any recognizable corticosteroids.