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A standardized method for the assay of small amounts of insulin (10–1000 µu./ml.) by measuring the effect on the glucose uptake of rat diaphragm in vitro is described. The most sensitive part of the concentration/response curve is within the range 10–150 µu./ml., and over this range there is a straight-line relationship between insulin concentration and insulin effect.

The importance of having a protein such as gelatin present in dilute solutions of insulin is discussed.