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Journal of Endocrinology (1961) 22, 31-46       DOI: 10.1677/joe.0.0220031
© 1961 Society for Endocrinology
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A METHOD FOR THE ANALYSIS OF URINARY 17-HYDROXYCORTICOSTEROIDS

J. D. FEW

A method for the analysis of urinary 17-hydroxycorticosteroids is described. The urinary steroids are submitted, in situ, to sodium borohydride reduction, sodium periodate oxidation and mild alkaline hydrolysis. The generated 17-ketosteroids are separated by partition chromatography into 11-deoxy and 11-oxy fractions which are separately estimated by the Zimmermann reaction. By this method it is possible to estimate the urinary metabolites of cortisol without interference by corticosteroids not oxygenated at C-11.




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C. Arvanitakis and R. F. Knouss
Selective Hypopituitarism: Impaired Cell-Mediated Immunity and Chronic Mucocutaneous Candidiasis
JAMA, September 17, 1973; 225(12): 1492 - 1495.
[Abstract] [PDF]




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