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¹ Lawson Health Research Institute, St Joseph's Health Care, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2 Departments of² Medicine³ Microbiology and Immunology⁴ , Physiology and Pharmacology⁵ Paediatrics, University of Western Ontario, London, Ontario, Canada N6A 5A5

(Correspondence should be addressed to D J Hill; Email: dhill{at}lri.sjhc.london.on.ca)

Dietary insult in early life can affect the development and future function of the endocrine pancreas. We maintained pregnant non-obese diabetic (NOD) mice on a low protein (LP, 8% protein versus control, 20%) diet from conception until the weaning of pups at day 21. Serum insulin and pancreatic insulin content were reduced in LP-fed NOD offspring at 8 weeks, as were serum interferon and pancreatic tumor necrosis factor , while the number of pancreatic islets demonstrating peri-insulitis, and the degree of invasiveness were reduced. To determine if LP caused early morphometric changes in the pancreas, we measured mean islet area at days 3 and 21. Mean islet size did not differ with diet, but by 8 weeks of age LP-fed NOD females exhibited a significantly reduced islet number and mean islet area, and a lower fractional area of pancreas occupied by both - and β-cells than control-fed mice. The onset of diabetes was delayed in NOD mice of both genders fed LP diet. The mechanism is likely to involve both altered β-cell morphology and function and changes in cytotoxic cytokines.